期刊
ORGANIC & BIOMOLECULAR CHEMISTRY
卷 14, 期 7, 页码 2186-2190出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5ob02609a
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资金
- NIGMS NIH HHS [R01 GM086703] Funding Source: Medline
Sirtuins are NAD-dependent lysine deacylases that play critical roles in cellular regulation and are implicated in human diseases. Modulators of sirtuins are needed as tools for investigating their biological functions and possible therapeutic applications. However, the discovery of sirtuin modulators is hampered by the lack of efficient sirtuin assays. Here we report an improved fluorogenic assay for SIRT1, SIRT2, and SIRT3 using a new substrate, a myristoyl peptide with a C-terminal aminocoumarin. The new assay has several advantages, including significantly lower substrate concentration needed, increased signal-to-background ratio, and improved Z'-factor. The novel assay thus will expedite high-throughput screening of SIRT1, SIRT2, and SIRT3 modulators.
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