期刊
OPTICS EXPRESS
卷 24, 期 25, 页码 28540-28548出版社
OPTICAL SOC AMER
DOI: 10.1364/OE.24.028540
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资金
- NASA Jet Propulsion Laboratory
- Gordon and Betty Moore Foundation [4037, 4038]
In situ investigation of microbial life in extreme environments can be carried out with microscopes capable of imaging 3-dimensional volumes and tracking particle motion. Here we present a lensless digital holographic microscope approach that provides roughly 1.5 micron resolution in a compact, robust package suitable for remote deployment. High resolution is achieved by generating high numerical-aperture input beams with radial gradient-index rod lenses. The ability to detect and track prokaryotes was explored using bacterial strains of two different sizes. In the larger strain, a variety of motions were seen, while the smaller strain was used to demonstrate a detection capability down to micron scales. (C) 2016 Optical Society of America
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