期刊
OPTICS EXPRESS
卷 24, 期 26, 页码 30038-30052出版社
OPTICAL SOC AMER
DOI: 10.1364/OE.24.030038
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资金
- Japan Society for the Promotion of Science (JSPS) (Program for World-Leading Innovative R & D on Science and Technology (FIRST), World Premier International Research Center Initiative Funding Program (WPI)) [KAKENHI JP15K17473]
- Japan Science and Technology (PRESTO)
- Grants-in-Aid for Scientific Research [15K17473] Funding Source: KAKEN
We present a measurement and reconstruction method for laser-scanning microscopy based on compressed sensing, which enables significantly higher frame rates and reduced photobleaching. The image reconstruction accuracy is ensured by including a model of the physical imaging process into the compressed sensing reconstruction procedure. We demonstrate its applicability to unmodified commercial confocal fluorescence microscopy systems and for Raman imaging, showing a potential data reduction of 10-15 times, which directly leads to improvements in acquisition speed, or reduction of photobleaching, without significant loss of spatial resolution. Furthermore, the reconstruction model is also robust to noise, and effective for low-light applications. This method has promising applications for all imaging modalities based on laser-scanning acquisition, including fluorescence, Raman, and nonlinear microscopy. (C) 2016 Optical Society of America
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