期刊
OPTICS EXPRESS
卷 24, 期 21, 页码 23719-23725出版社
OPTICAL SOC AMER
DOI: 10.1364/OE.24.023719
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资金
- National Science Foundation (NSF) [DMR-1306410, DMR-1420570]
- Army Research Office MURI [W911NF-13-1-0383]
- Direct For Mathematical & Physical Scien
- Division Of Materials Research [1306410] Funding Source: National Science Foundation
We use in-line digital holographic microscopy to image freely swimming E. coli. We show that fitting a light scattering model to E. coli holograms can yield quantitative information about the bacterium's body rotation and tumbles, offering a precise way to track fine details of bacterial motility. We are able to extract the cell's three-dimensional (3D) position and orientation and recover behavior such as body angle rotation during runs, tumbles, and pole reversal. Our technique is label-free and capable of frame rates limited only by the camera. (C) 2016 Optical Society of America
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