期刊
OPTICS AND LASERS IN ENGINEERING
卷 80, 期 -, 页码 24-31出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.optlaseng.2015.12.012
关键词
Microscopy; Phase retrieval; Multi-modal imaging; Quantitative phase microscopy; Light field microscopy
类别
资金
- National Natural Science Fund of China [11574152, 61505081]
- Six Talent Peaks project (Jiangsu Province, China) [2015-DZXX-009]
- '333 Engineering' research project (Jiangsu Province, China) [BRA2015294]
- Fundamental Research Funds for the Central Universities [30915011318]
- Open Research Fund of Jiangsu Key Laboratory of Spectral Imaging & Intelligent Sense [3092014012200417]
- Nanjing University of Science and Technology
We demonstrate a simple and cost-effective programmable aperture microscope to realize multi-modal computational imaging by integrating a programmable liquid crystal display (LCD) into a conventional wide-field microscope. The LCD selectively modulates the light distribution at the rear aperture of the microscope objective, allowing numerous imaging modalities, such as bright field, dark field, differential phase contrast, quantitative phase imaging, multi-perspective imaging, and full resolution light field imaging to be achieved and switched rapidly in the same setup, without requiring specialized hardwares and any moving parts. We experimentally demonstrate the success of our method by imaging unstained cheek cells, profiling microlens array, and changing perspective views of thick biological specimens. The post-exposure refocusing of a butterfly mouthpart and REP-labeled dicot stem cross-section is also presented to demonstrate the full resolution light field imaging capability of our system for both translucent and fluorescent specimens. (C) 2015 Elsevier Ltd. All rights reserved.
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