期刊
ONCOGENE
卷 36, 期 20, 页码 2900-2909出版社
SPRINGERNATURE
DOI: 10.1038/onc.2016.446
关键词
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资金
- China International Science and Technology Cooperation Program [2015DFM30040]
- National Science and Technology Major Project [2015ZX09101009]
- National Natural Science Foundation of China [81573464, 81321092]
- NSFC-Shandong Joint Fund for Marine Science Research Centers [U1406402]
Phosphoglycerate mutase 1 (PGAM1) is a glycolytic enzyme that coordinates glycolysis and biosynthesis to promote cancer growth via its metabolic activity. Here, we report the discovery of a non-metabolic function of PGAM1 in promoting cancer metastasis. A proteomic study identified a-smooth muscle actin (ACTA2) as a PGAM1-associated protein. PGAM1 modulated actin filaments assembly, cell motility and cancer cell migration via directly interacting with ACTA2, which was independent of its metabolic activity. The enzymatically inactive H186R mutant retained its association with ACTA2, whereas 201-210 amino acids deleted PGAM1 mutant lost the interaction with ACTA2 regardless of intact metabolic activity. Importantly, PGAM1 knockdown decreased metastatic potential of breast cancer cells in vivo and PGAM1 and ACTA2 were jointly associated with the prognosis of breast cancer patients. Together, this study provided the first evidence revealing a non-metabolic function of PGAM1 in promoting cell migration, and gained new insights into the role of PGAM1 in cancer progression.
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