期刊
NMR IN BIOMEDICINE
卷 29, 期 11, 页码 1656-1665出版社
WILEY-BLACKWELL
DOI: 10.1002/nbm.3613
关键词
acquisition methods; MEGA editing; MRS and MRSI methods; neurotransmitters; normal brain; reproducibility
资金
- Austrian Science Fund (FWF) [KLI-61]
- FFG Bridge Early Stage Grant [846505]
- project SAS-NSC Joint Research Cooperation Grant [2013/17]
- project VEGA [2/0191/15]
- project Increasing opportunities for career growth in research and development in the field of medical sciences [ITMS: 26110230067]
- project Biomedical Center Martin - EU [ITMS: 26220220187]
- Austrian Science Fund (FWF) [KLI61] Funding Source: Austrian Science Fund (FWF)
- Austrian Science Fund (FWF) [KLI 61] Funding Source: researchfish
The reproducibility of gamma-aminobutyric acid (GABA) quantification results, obtained with MRSI, was determined on a 3T MR scanner in healthy adults. In this study, a spiral-encoded, GABA-edited, MEGA-LASER MRSI sequence with real-time motion-scanner-instability corrections was applied for robust 3D mapping of neurotransmitters in the brain. In particular, the GABA(+) (i.e. GABA plus macromolecule contamination) and Glx (i.e. glutamate plus glutamine contamination) signal was measured. This sequence enables 3D-MRSI with about 3cm(3) nominal resolution in about 20min. Since reliable quantification of GABA is challenging, the spatial distribution of the inter-subject and intra-subject variability of GABA(+) and Glx levels was studied via test-retest assessment in 14 healthy volunteers (seven men-seven women). For both inter-subject and intra-subject repeated measurement sessions a low coefficient of variation (CV) and a high intraclass correlation coefficient (ICC) were found for GABA(+) and Glx ratios across all evaluated voxels (intra-/inter-subject: GABA(+) ratios, CV similar to 8%-ICC>0.75; Glx ratios, CV similar to 6%-ICC>0.70). The same was found in selected brain regions for Glx ratios versus GABA(+) ratios (CV varied from about 5% versus about 8% in occipital and parietal regions, to about 8% versus about 10% in the frontal area, thalamus, and basal ganglia). These results provide evidence that 3D mapping of GABA(+) and Glx using the described methodology provides high reproducibility for application in clinical and neuroscientific studies.
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