期刊
NEW PHYTOLOGIST
卷 213, 期 4, 页码 1755-1770出版社
WILEY
DOI: 10.1111/nph.14317
关键词
antioxidant; auxin; catechol; Nicotiana attenuata; post-fire annual; reactive oxygen species (ROS); smoke
资金
- Max Planck Society
- European Research Council to ITB [293926]
- Collaborative Research Centre 'Chemical Mediators in Complex Biosystems-ChemBioSys' [SFB 1127]
- SNF [PEBZP3-142886]
- Marie Curie Intra-European-Fellowship [328935]
- European Research Council (ERC) [293926] Funding Source: European Research Council (ERC)
Nicotiana attenuata germinates from long-lived seedbanks in native soils after fires. Although smoke signals have been known to break seed dormancy, whether they also affect seedling establishment and root development remains unclear. In order to test this, seedlings were treated with smoke solutions. Seedlings responded in a dose-dependent manner with significantly increased primary root lengths, due mainly to longitudinal cell elongation, increased numbers of lateral roots and impaired root hair development. Bioassay-driven fractionations and NMR were used to identify catechol as the main active compound for the smoke-induced root phenotype. The transcriptome analysis revealed that mainly genes related to auxin biosynthesis and redox homeostasis were altered after catechol treatment. However, histochemical analyses of reactive oxygen species (ROS) and the inability of auxin applications to rescue the phenotype clearly indicated that highly localized changes in the root's redox-status, rather than in levels of auxin, are the primary effector. Moreover, H2O2 application rescued the phenotype in a dose-dependent manner. Chemical cues in smoke not only initiate seed germination, but also influence seedling root growth; understanding how these cues work provides new insights into the molecular mechanisms by which plants adapt to post-fire environments.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据