期刊
NEW PHYTOLOGIST
卷 213, 期 3, 页码 1052-1067出版社
WILEY
DOI: 10.1111/nph.14267
关键词
conserved oligomeric Golgi (COG) complex; exocyst; microtubules; secondary cell wall; tracheary elements; xylem
资金
- Czech Science Foundation (GACR) [15-14886S]
- Grant Agency of Charles University (GAUK) [1230214]
- MEXT KAKENHI [26113505, 15H01243]
- JSPS KAKENHI [25440128]
- Mitsubishi Foundation
- Ministry of Education Youth and Sport of the Czech Republic [NPUILO1417]
- Grants-in-Aid for Scientific Research [16H01247, 25440128, 15H01243, 26113505, 16H06172] Funding Source: KAKEN
Cortical microtubules (MTs) play a major role in the patterning of secondary cell wall (SCW) thickenings in tracheary elements (TEs) by determining the sites of SCW deposition. The EXO70A1 subunit of the exocyst secretory vesicle tethering complex was implicated to be important for TE development via the MT interaction. We investigated the subcellular localization of several exocyst subunits in the xylem of Arabidopsis thaliana and analyzed the functional significance of exocyst-mediated trafficking in TE development. Live cell imaging of fluorescently tagged exocyst subunits in TE using confocal microscopy and protein-protein interaction assays were performed to describe the role of the exocyst and its partners in TE development. In TEs, exocyst subunits were localized to the sites of SCW deposition in an MT-dependent manner. We propose that the mechanism of exocyst targeting to MTs involves the direct interaction of exocyst subunits with the COG2 protein. We demonstrated the importance of a functional exocyst subunit EXO84b for normal TE development and showed that the deposition of SCW constituents is partially compromised, possibly as a result of the mislocalization of secondary cellulose synthase in exocyst mutants. We conclude that the exocyst complex is an important factor bridging the pattern defined by cortical MTs with localized secretion of the SCW in developing TEs.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据