期刊
NEW PHYTOLOGIST
卷 212, 期 1, 页码 161-175出版社
WILEY
DOI: 10.1111/nph.14013
关键词
coiled-coil (CC); Nicotiana benthamiana; N-terminal domain; nucleotide-binding leucine-rich repeat (NB-LRR); resistance protein; Solanum lycopersicum; Tomato spotted wilt tospovirus
资金
- National Natural Science Foundation of China [31222045, 31471746]
- National Program on Key Basic Research Project of China (973 Program) [2014CB138400]
- Youth Talent Support Program of China
- Distinguished Professor of Jiangsu Province
The tomato resistance protein Sw-5b differs from the classical coiled-coil nucleotide-binding leucine-rich repeat (CC-NB-LRR) resistance proteins by having an extra N-terminal domain (NTD). To understand how NTD, CC and NB-LRR regulate autoinhibition and activation of Sw-5b, we dissected the function(s) of each domain. When viral elicitor was absent, Sw-5b LRR suppressed the central NB-ARC to maintain autoinhibition of the NB-LRR segment. The CC and NTD domains independently and additively enhanced the autoinhibition of NB-LRR. When viral elicitor was present, the NB-LRR segment of Sw-5b was specifically activated to trigger a hypersensitive response. Surprisingly, Sw-5b CC suppressed the activation of NB-LRR, whereas the extra NTD of Sw-5b became a positive regulator and fully activated the resistance protein, probably by relieving the inhibitory effects of the CC. In infection assays of transgenic plants, the NB-LRR segment alone was insufficient to confer resistance against Tomato spotted wilt tospovirus; the layers of NTD and CC regulation on NB-LRR were required for Sw-5b to confer resistance. Based on these findings, we propose that, to counter the negative regulation of the CC on NB-LRR, Sw-5b evolved an extra NTD to coordinate with the CC, thus developing a multilayered regulatory mechanism to control autoinhibition and activation.
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