期刊
NEW PHYTOLOGIST
卷 210, 期 4, 页码 1298-1310出版社
WILEY
DOI: 10.1111/nph.13860
关键词
epigenetics; fiber development; Gossypium hirsutum (cotton); MYBMIXTA-like (MML) transcription factor; natural antisense transcripts (NATs); small interfering RNAs (siRNAs)
Natural antisense transcripts (NATs) are commonly observed in eukaryotic genomes, but only a limited number of such genes have been identified as being involved in gene regulation in plants. In this research, we investigated the function of small RNA derived from a NAT in fiber cell development. Using a map-based cloning strategy for the first time in tetraploid cotton, we cloned a naked seed mutant gene (N-1) encoding a MYBMIXTA-like transcription factor 3 (MML3)/GhMYB25-like in chromosome A12, GhMML3_A12, that is associated with fuzz fiber development. The extremely low expression of GhMML3_A12 in N-1 is associated with NAT production, driven by its 3' antisense promoter, as indicated by the promoter-driven histochemical staining assay. In addition, small RNA deep sequencing analysis suggested that the bidirectional transcriptions of GhMML3_A12 form double-stranded RNAs and generate 21-22 nt small RNAs. Therefore, in a fiber-specific manner, small RNA derived from the GhMML3_A12 locus can mediate GhMML3_A12 mRNA self-cleavage and result in the production of naked seeds followed by lint fiber inhibition in N-1 plants. The present research reports the first observation of gene-mediated NATs and siRNA directly controlling fiber development in cotton.
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