期刊
NEW PHYTOLOGIST
卷 212, 期 1, 页码 42-50出版社
WILEY-BLACKWELL
DOI: 10.1111/nph.14107
关键词
Arabidopsis development; auxin response factor (ARF); embryogenesis; organogenesis; PIN FORMED genes; root apical meristem; self-organized patterning; shoot apical meristem
资金
- Natural Sciences and Engineering Research Council of Canada
- Center for Analysis of Genome Evolution and Function (CAGEF)
- National Institute of General Medical Sciences of the National Institutes of Health [R15GM114733]
The regulatory effect auxin has on its own transport is critical in numerous self-organizing plant patterning processes. However, our understanding of the molecular mechanisms linking auxin signal transduction and auxin transport is still fragmentary, and important regulatory genes remain to be identified. To track a key link between auxin signaling and auxin transport in development, we established an Arabidopsis thaliana genetic background in which fundamental patterning processes in both shoot and root were essentially abolished and the expression of PIN FORMED (PIN) auxin efflux facilitators was dramatically reduced. In this background, we demonstrate that activating a steroid-inducible variant of the auxin response factor (ARF) MONOPTEROS (MP) is sufficient to restore patterning and PIN gene expression. Further, we show that MP binds to distinct promoter elements of multiple genetically defined PIN genes. Our work identifies a direct regulatory link between central, well-characterized genes involved in auxin signal transduction and auxin transport. The steroid-inducible MP system directly demonstrates the importance of this molecular link in multiple patterning events in embryos, shoots and roots, and provides novel options for interrogating the properties of self-regulated auxin-based patterning in planta.
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