期刊
NEW PHYTOLOGIST
卷 211, 期 2, 页码 516-526出版社
WILEY
DOI: 10.1111/nph.13903
关键词
hypersensitive response; nitric oxide (NO); plant disease resistance; plant immunity; post-translational modifications; redox signalling; S-nitrosylation and S-nitrosothiols
资金
- Biotechnology and Biological Sciences Research Council (BBSRC)
- Royal Society Research Fellowship [UF090321, Rg110495]
- BBSRC research grant [BB/DO11809/1]
- Global Partnership Fund [14-174]
- Next-Generation Biogreen 21 program (SSAC), Rural Development Administration, Republic of Korea [PJ01110201]
- Biotechnology and Biological Sciences Research Council [BB/H000984/1, BB/D011809/1] Funding Source: researchfish
- BBSRC [BB/D011809/1, BB/H000984/1] Funding Source: UKRI
Nitric oxide (NO) is emerging as a key regulator of diverse plant cellular processes. A major route for the transfer of NO bioactivity is S-nitrosylation, the addition of an NO moiety to a protein cysteine thiol forming an S-nitrosothiol (SNO). Total cellular levels of protein S-nitrosylation are controlled predominantly by S-nitrosoglutathione reductase 1 (GSNOR1) which turns over the natural NO donor, S-nitrosoglutathione (GSNO). In the absence of GSNOR1 function, GSNO accumulates, leading to dysregulation of total cellular S-nitrosylation. Here we show that endogenous NO accumulation in Arabidopsis, resulting from loss-of-function mutations in NO Overexpression 1 (NOX1), led to disabled Resistance (R) gene-mediated protection, basal resistance and defence against nonadapted pathogens. In nox1 plants both salicylic acid (SA) synthesis and signalling were suppressed, reducing SA-dependent defence gene expression. Significantly, expression of a GSNOR1 transgene complemented the SNO-dependent phenotypes of paraquat resistant 2-1 (par2-1) plants but not the NO-related characters of the nox1-1 line. Furthermore, atgsnor1-3 nox1-1 double mutants supported greater bacterial titres than either of the corresponding single mutants. Our findings imply that GSNO and NO, two pivotal redox signalling molecules, exhibit additive functions and, by extension, may have distinct or overlapping molecular targets during both immunity and development.
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