期刊
FASEB BIOADVANCES
卷 -, 期 -, 页码 -出版社
WILEY
DOI: 10.1096/fba.2023-00070
关键词
extracellular vesicles; liquid biomarker; mitochondrial DNA; mutation; triple-negative breast cancer
This study aimed to develop mitochondrial DNA (mtDNA)-based liquid biomarkers for early detection and recurrence prediction in triple-negative breast cancer (TNBC) patients. The study identified hotspot mtDNA mutations in serum-derived extracellular vesicles (EVs) of TNBC patients, which could be potentially used for noninvasive detection. Additionally, the study found that women with African ancestry had higher mtDNA mutation load. The detection of tumor-specific mtDNA mutations and measurement of mtDNA and cardiolipin (CL) contents in EVs could be valuable for noninvasive early detection and recurrence prediction strategies.
Early detection and recurrence prediction are challenging in triple-negative breast cancer (TNBC) patients. We aimed to develop mitochondrial DNA (mtDNA)-based liquid biomarkers to improve TNBC management. Mitochondrial genome (MG) enrichment and next-generation sequencing mapped the entire MG in 73 samples (64 tissues and 9 extracellular vesicles [EV] samples) from 32 metastatic TNBCs. We measured mtDNA and cardiolipin (CL) contents, NDUFB8, and SDHB protein expression in tumors and in corresponding circulating EVs. We identified 168 nonsynonymous mtDNA mutations, with 73% (123/186) coding and 27% (45/168) noncoding in nature. Twenty percent of mutations were nucleotide transversions. Respiratory complex I (RCI) was the key target, which harbored 44% (74/168) of the overall mtDNA mutations. A panel of 11 hotspot mtDNA mutations was identified among 19%-38% TNBCs, which were detectable in the serum-derived EVs with 82% specificity. Overall, 38% of the metastatic tumor-signature mtDNA mutations were traceable in the EVs. An appreciable number of mtDNA mutations were homoplasmic (18%, 31/168), novel (14%, 23/168), and potentially pathogenic (9%, 15/168). The overall and RCI-specific mtDNA mutational load was higher in women with African compared to European ancestry accompanied by an exclusive abundance of respiratory complex (RC) protein NDUFB8 (RCI) and SDHB (RCII) therein. Increased mtDNA (p < 0.0001) content was recorded in both tumors and EVs along with an abundance of CL (p = 0.0001) content in the EVs. Aggressive tumor-signature mtDNA mutation detection and measurement of mtDNA and CL contents in the EVs bear the potential to formulate noninvasive early detection and recurrence prediction strategies.
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