Advances and Opportunities in H2S Measurement in Chemical Biology

Hydrogen sulfide (H2S) is an important biological mediator across all kingdoms of life and plays intertwined roles in various disciplines, ranging from geochemical cycles to industrial processes. A common need across these broad disciplines is the ability to detect and measure H2S in complex sample environments. This Perspective focuses on key advances and opportunities for H2S detection and quantification that are relevant to chemical biology. Specifically, we focus on methods for H2S detection and quantification most commonly used in biological samples, including activity-based H2S probes, the methylene blue assay, the monobromobimane assay, and H2S-sensitive electrode measurements. Our goal is to help simplify what at first may seem to be an overwhelming array of detection and measurement choices, to articulate the strengths and limitations of individual techniques, and to highlight key unmet needs and opportunities in the field.

Automated summary

This Perspective paper discusses the important advances and opportunities in the detection and quantification of H2S, focusing on the field of chemical biology. It presents commonly used methods for H2S detection and quantification in biological samples, highlighting the strengths and limitations of each technique. The goal of the paper is to simplify the decision-making process and identify key unmet needs and opportunities in the field.