期刊
NEUROTOXICOLOGY AND TERATOLOGY
卷 57, 期 -, 页码 30-38出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.ntt.2016.05.001
关键词
Polycyclic aromatic hydrocarbon (PAH); Nitrated polycyclic aromatic hydrocarbon (NPAH); Polystyrene; Sorption; 96-Well plate
资金
- National Institute of Environmental Health Sciences (NIEHS) Graduate Student T32 Training Grant [3 T32 ES000760]
- NIEHS [P30 ES025128]
- Oregon State University Superfund Research Program NIEHS [P42 ES016465]
- Ruth L. Kirschstein National Research Service Award [F31 ES026037-01]
Developing zebrafish are increasingly being used for rapid assessments of chemical toxicity, and these assays are frequently conducted in multi-well plastic plates. This study investigated the sorptive behavior of polycyclic aromatic hydrocarbons (PAHs) and nitrated PAHs (NPAHs) to uncoated 96-well polystyrene plates typically used for zebrafish (Danio rerio) testing. We measured the percent sorption in the presence and absence of zebrafish embryos, at two exposure concentrations, as well as using two-different procedures (addition of embryos to polystyrene plates either before analyte addition, or allowing 24 h of equilibrium between analyte addition and embryo addition to the polystyrene plates). Following exposure, the plates were extracted with hexane and analyzed using gas chromatography coupled with mass spectrometry (GC/MS). Allowing 24 h of pre-incubation between the addition of analytes and embryos did not significantly impact the percent sorption. The percent sorption was higher for both PAHs and NPAHs at the lower exposure concentration, and sorption was lower in the presence of zebrafish embryos. A mass balance model was developed to predict the sorption to polystyrene plates, based on the PAH and NPAH mass distribution ratios between polystyrene and water. While PAH sorption was significantly correlated with subcooled liquid solubility, NPAH sorption did not correlate with any of the physical-chemical properties investigated. This indicates the need to better understand the sorptive behavior of hydrophobic analytes to plastics, and to better account for sorptive losses during toxicity testing in polystyrene plates. (C) 2016 Published by Elsevier Inc.
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