期刊
NEUROSCIENCE LETTERS
卷 626, 期 -, 页码 48-53出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.neulet.2016.05.021
关键词
Astrocyte; Neuron; Reactive astrocytosis; Water homeostasis
资金
- JSPS KAKENHI Grant [26461314]
- Brain Research Institute, University of Niigata [2224]
- Grants-in-Aid for Scientific Research [26461314] Funding Source: KAKEN
Aquaporin-1 (AQPI) is a water channel expressed in the choroid plexus and participates in forming cerebrospinal fluid. Interestingly, reactive astrocytes also express AQPI in the central nervous system under some pathological conditions. On the other hand, 3-nitropropionic acid (3NP) is a mitochondrial toxin that causes selective degeneration of striatum; however, its chemical preconditioning is neuroprotective against cerebral ischemia. We previously reported that mild 3NP application is accompanied with numerous reactive astrocytes in rat striatum devoid of typical necrotic lesions. Therefore, we studied whether AQPI in the rat striatum could be upregulated with reactive astrocytosis using the 3NP model. Immunohistochemical or immunofluorescence analysis showed that reactive astrocytosis in the striatum, which upregulates glial fibrillary acidic protein and glutamine synthetase, was induced by mild doses of 3NP administration. Intriguingly, after 3NP treatment, AQPI was intensely expressed not only by the subpopulation of astroglia but also by neurons. The AQPI immunoreactivity became more intensified at the early-subtoxic stage (ES: 24-48 h), but not as much in the delayed-subtoxic stage (DS: 96-120 h). In contrast, AQP4 expression in the striatum was downregulated after 3NP treatment, in particular during the ES stage. AQPI upregulation/AQP4 downregulation induced under subtoxic 3NP treatment may play a pivotal role in water homeostasis and cell viability in the striatum. (C) 2016 Elsevier Ireland Ltd. All rights reserved.
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