期刊
BIOENGINEERING-BASEL
卷 10, 期 8, 页码 -出版社
MDPI
DOI: 10.3390/bioengineering10080888
关键词
guided bone regeneration; clot stability; static magnetic field; trypsin; fibrinolysis; tissue-type plasminogen activator; MG63 osteoblast-like cells
The influence of a magnetic field on the activation of bone cells and remodelling of alveolar bone has been studied, but its effect on blood clot dissolution has not yet been investigated. This study found that a static magnetic field can increase the stability of blood clots when tissue factor and t-PA + plasminogen are used. However, the magnetic field did not have a significant effect on cell proliferation. Further research is needed to fully utilize magnetic forces in guided bone regeneration for orthodontics and prosthodontics.
Background: The influence of a magnetic field on the activation of bone cells and remodelling of alveolar bone is known to incite bone regeneration. Guided Bone Regeneration (GBR) aims to develop biomimetic scaffolds to allow for the functioning of the barrier and the precise succession of wound healing steps, including haemostasis. The effect of a magnetic field on blood clot dissolution has not been studied yet. Methods: We conducted a methodological study on the clot stability in the presence of a static magnetic field (SMF). Preformed whole blood (WB) clots were treated with either a broad proteolytic enzyme (trypsin) or a specific fibrinolytic agent, i.e., tissue-type plasminogen activator (t-PA). MG63 osteoblast-like cells were added to preformed WB clots to assess cell proliferation. Results: After having experienced a number of clotting and dissolution protocols, we obtained clot stability exerted by SMF when tissue factor (for clotting) and t-PA + plasminogen (for fibrinolysis) were used. WB clots allowed osteoblast-like cells to survive and proliferate, however no obvious effects of the magnetic field were noted. Conclusions: Paramagnetic properties of erythrocytes may have influenced the reduction in clot dissolution. Future studies are warranted to fully exploit the combination of magnetic forces, WB clot and cells in GBR applied to orthodontics and prosthodontics.
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