期刊
NEURON
卷 90, 期 3, 页码 492-498出版社
CELL PRESS
DOI: 10.1016/j.neuron.2016.03.013
关键词
-
资金
- Heisenberg Program of the German Research Foundation [HA 6386/2-2, 3-2]
- Leibniz-Visiting Scientist program
- NIDCD grant [DC012938]
The fusion of neurotransmitter-filled vesicles during synaptic transmission is balanced by endocytotic membrane retrieval. Despite extensive research, the speed and mechanisms of synaptic vesicle endocytosis have remained controversial. Here, we establish low-noise time-resolved membrane capacitance measurements that allow monitoring changes in surface membrane area elicited by single action potentials and stronger stimuli with high-temporal resolution at physiological temperature in individual bona-fide mature central synapses. We show that single action potentials trigger very rapid endocytosis, retrieving presynaptic membrane with a time constant of 470 ms. This fast endocytosis is independent of clathrin but mediated by dynamin and actin. In contrast, stronger stimuli evoke a slower mode of endocytosis that is clathrin, dynamin, and actin dependent. Furthermore, the speed of endocytosis is highly temperature dependent with a Q(10) of similar to 3.5. These results demonstrate that distinct molecular modes of endocytosis with markedly different kinetics operate at central synapses.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据