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Construction and characterization of a saturated Tn-seq library of Salmonella Typhimurium ATCC 14028

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AMER SOC MICROBIOLOGY
DOI: 10.1128/MRA.00365-23

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Salmonella Typhimurium; Tn-seq; transposons; virulence plasmid; essential genes; TRANSIT; growth advantage; growth disadvantage

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In this study, a high-density transposon sequencing library of the Salmonella Typhimurium ATCC 14028 strain was constructed and characterized, and essential genes for growth in rich culture medium were identified.
Salmonella enterica is an important foodborne pathogen. Here, we present the construction and characterization of a high-density transposon sequencing library of the Salmonella Typhimurium ATCC 14028 strain. Essential, advantageous, and disadvantageous genes for growth in rich culture medium were identified on the chromosome and the pSLT plasmid.

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