HPLC-DAD optimization of quantification of vescalagin, gallic and ellagic acid in chestnut tannins

The quantification of hydrolysable polyphenols such as gallic, ellagic acid and vescalagin by HPLC-DAD is classically run after methanol extraction as a reference solvent. Water extraction is usually discarded because of a lower obtention of total polyphenol content compared to methanol extraction. In our study, methanol was compared to water extraction in both the total polyphenol content method and the HPLC-DAD analysis. Total polyphenol content in water extraction was lower than in methanol extraction, but water extraction gave better results on HPLC-DAD. In conclusion, total polyphenol content cannot be used as reference to choose the solvent of extraction to quantify some polyphenols by HPLC-DAD because of the specific properties of each polyphenol. Indeed, recovery results obtained on hydrolysable polyphenols with water extraction were better and with a lower variability than following methanol extraction.

Automated summary

This study compared the effectiveness of methanol and water extraction methods in total polyphenol content and HPLC-DAD analysis. The results showed that water extraction had lower total polyphenol content compared to methanol extraction, but yielded better results in HPLC-DAD analysis. Therefore, total polyphenol content cannot be used as a reference to determine the extraction solvent due to the specific properties of each polyphenol.