Rapidly in situ forming an injectable Chitosan/PEG hydrogel for intervertebral disc repair

Intervertebral disc (IVD) degeneration occurred with the increasing age or accidents has puzzled peoples in daily life. To seal IVD defect by injectable hydrogels is a promising method for slowing down IVD degeneration. Herein, we reported a rapidly in situ forming injectable chitosan/PEG hydrogel (CSMA-PEGDA-L) through integrating photo-crosslink of methacrylate chitosan (CSMA) with Schiff base reaction between CSMA and aldehyde polyethylene glycol (PEGDA). The CSMA-PEGDA-L possessed a stronger compressive strength than the photo-crosslinked CSMA-L hydrogel and Schiff-base-crosslinked CSMA-PEGDA hydrogel. This chitosan/PEG hydrogel showed low cytotoxicity from incubation experiments of nucleus pulpous cells. When implanted on the punctured IVD of rat's tail, the CSMA-PEGDA-L hydrogel could well retard the progression of IVD degeneration through physical plugging, powerfully proven by radiological and histological evaluations. This work demon-strated the strategy of in situ injectable glue may be a potential solution for prevention of IVD degeneration.

Automated summary

This study reported a rapidly forming injectable chitosan/PEG hydrogel (CSMA-PEGDA-L) that can retard the progression of intervertebral disc (IVD) degeneration. The hydrogel demonstrated strong compressive strength and low cytotoxicity. When implanted on the punctured IVD of rats, it effectively slowed down the degenerative changes.