期刊
HORTICULTURAE
卷 9, 期 8, 页码 -出版社
MDPI
DOI: 10.3390/horticulturae9080884
关键词
CRISPR-Cas9; crop improvement; genome editing; horticultural crops; TALENs; ZFNs
类别
Genome editing is a powerful tool for precise genetic modifications in horticultural crops, with CRISPR-Cas9, TALENs, and ZFNs being the major editing tools discussed in this review. CRISPR-Cas9 has the potential to enhance disease resistance, stress tolerance, nutritional content, and yield in horticultural crops. TALENs and ZFNs offer alternative options for targeted DNA modifications and have shown success in specific applications. The review emphasizes the benefits of genome editing in horticulture, while acknowledging the challenges of off-target effects, delivery methods, and regulatory frameworks. This technology can contribute to sustainable advancements in horticulture, benefiting both producers and consumers worldwide.
Genome editing has emerged as a powerful tool for accelerating crop improvement in horticultural crops by enabling precise modifications to their genetic makeup. This review provides an in-depth exploration of the applications, methodologies, and potential impacts of genome editing in horticulture. The review focuses on three major genome editing tools in horticulture, CRISPR-Cas9, TALENs, and ZFNs. The underlying mechanisms, applications, and potential challenges associated with each tool are discussed in detail. CRISPR-Cas9, being a versatile and widely used system, has the potential to enhance traits such as disease resistance, abiotic stress tolerance, nutritional content, and yield in horticultural crops. TALENs and ZFNs, although less commonly used, offer alternative options for targeted DNA modifications, and have demonstrated success in specific applications. We emphasize the potential benefits of genome editing in horticulture, including improved crop productivity, quality, and nutritional value. However, challenges such as off-target effects, delivery methods, and regulatory frameworks need to be addressed for the full realization of this technology's potential. This review serves as a valuable resource for researchers, policymakers, and stakeholders, providing insights into the opportunities and complexities associated with harnessing genome editing for enhanced traits in horticultural crops. By navigating these challenges, genome editing can contribute to sustainable advancements in horticulture, benefiting both producers and consumers worldwide.
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