期刊
VETERINARY SCIENCES
卷 10, 期 8, 页码 -出版社
MDPI
DOI: 10.3390/vetsci10080520
关键词
Sarcocystis; small ruminants; intermediate host-specificity; cox1; phylogeny; microscopy
Contradictory data on intermediate host specificity of Sarcocystis spp. in farm animals exists. This study molecularly tested samples from sheep and goats in Lithuania to identify Sarcocystis species described in other intermediate hosts but suspected to be non-canonical parasites to these small ruminants. The findings revealed the presence of previously thought to be host-specific Sarcocystis species in different hosts, highlighting the need for further research.
Contradictory data is available on the intermediate host specificity of Sarcocystis spp. in farm animals. Therefore, the current work aimed at molecularly testing samples of sheep and goats reared in Lithuania to identify Sarcocystis species described in other intermediate hosts but suspected to be non-canonical parasites to these small ruminants. For this purpose, muscle samples from 47 domestic sheep and nine goats were examined. Sarcocystis species were identified using direct and nested PCR targeting cox1 and sequencing of positive amplified products. Along with the detection of the canonical Sarcocystis spp. in their respective intermediate hosts, the DNA of S. capracanis and S. morae was detected in sheep, although these species were previously thought to be specific to goats and deer, respectively. In addition, DNA from S. arieticanis and S. tenella was found in goats, even though these two species were believed to be sheep-specific. Notably, under light microscopy, only sarcocysts of S. capracanis specific to goats were observed. Thus, future research on the life cycle and host-specificity of Sarcocystis spp. examined is warranted.
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