期刊
FERMENTATION-BASEL
卷 9, 期 7, 页码 -出版社
MDPI
DOI: 10.3390/fermentation9070612
关键词
& gamma;-aminobutyric acid; thin layer chromatography; anti-inflammatory; probiotics; lactic acid bacteria
In this study, four Levilactobacillus brevis strains with high ?-aminobutyric acid (GABA) production ability were selected from 33 strains isolated from diverse fermented foods in South Korea. The GABA-producing ability of these strains was evaluated using thin-layer chromatography, and the GABA levels were measured using an amino acid analyzer. Furthermore, the anti-inflammatory activity of the selected strains was investigated, and the results demonstrated that the cell-free supernatant of these strains could reduce nitric oxide (NO) production, inducible nitric oxide synthase (iNOS) expression, and nuclear factor kappa B (NF-?B) activity in RAW264.7 macrophages. Therefore, these GABA-producing LAB strains can modulate neuronal excitation and exhibit probiotic properties with anti-inflammatory activity.
Lactic acid bacteria have become popular because of their ?-aminobutyric acid (GABA)-producing ability. In the present study, we selected four Levilactobacillus brevis strains (MG5552, MG5405, MG5261, and MG5522) with GABA-producing ability from the 33 strains isolated from various fermented foods in South Korea. We evaluated their GABA-producing ability using thin-layer chromatography and determined the GABA levels produced by each strain using an amino acid analyzer. Moreover, we investigated the anti-inflammatory activity of the selected strains, and the results revealed that the cell-free supernatant of the strains decreased nitric oxide (NO), inducible nitric oxide synthase (iNOS) expression, and nuclear factor kappa B (NF-?B) activity in RAW264.7 macrophages. Therefore, these GABA-producing LAB strains can regulate nerve excitement and act as probiotics with anti-inflammatory activity.
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