Composites of Fe3O4, Ag, and Au Nanoparticles for Dye Degradation and Selective Detection of Glutathione

In comparison with the poor stability, high cost, anddifficultpreparation of conventional natural enzymes, nanozymes have the obviousadvantages of excellent stability, high catalytic activity, multifunctionality,and large-scale preparation. Herein, a series of Fe3O4 NPs-based magnetic composite with dual enzyme-like activities(peroxidase and oxidase) were successfully synthesized by the microwave-assistedmethod. Among them, the Fe3O4/Ag Au nanocompositeshowed the highest peroxidase-like activity due to the synergisticcatalytic effect of Ag and Au bimetallic modifications. Enzyme kineticstudies revealed good kinetic parameters of the Fe3O4/Ag Au nanocomposite for peroxidase, and it also exhibiteda higher affinity for TMB compared to natural horseradish peroxidase.In addition, a method for effective dye degradation and selectivedetection of glutathione was constructed based on its peroxidase-likeactivity. Importantly, the remarkable magnetic separation propertyand the outstanding stability proved the extensive application prospectsof the nanozymes in the remediation of water pollution and some biomarker-specificdetection.

Automated summary

Compared to conventional natural enzymes, nanozymes have significant advantages including excellent stability, high catalytic activity, multifunctionality and large-scale preparation. In this study, Fe3O4 NPs-based magnetic composites with dual enzyme-like activities were synthesized successfully using a microwave-assisted method. Among them, Fe3O4/Ag Au nanocomposite exhibited the highest peroxidase-like activity due to the synergistic effect of Ag and Au bimetallic modifications. The nanozymes show great potential in the remediation of water pollution and biomarker-specific detection due to their remarkable magnetic separation property and outstanding stability.