期刊
LIFE-BASEL
卷 13, 期 8, 页码 -出版社
MDPI
DOI: 10.3390/life13081733
关键词
food allergy; food matrix; allergic reaction; ovalbumin
The search for an animal model to assess the allergenic potential of processed food products is ongoing. Researchers evaluated sensitization to ovalbumin (OVA) in different structural states and the allergic response triggered after intra-gastric or food challenges. The proposed murine model of egg allergy holds the potential for evaluating the impact of food matrix composition and processing on the threshold of egg-allergic responses.
The search for an animal model to evaluate the allergenic potential of processed food products is still ongoing. Both the sensitization to ovalbumin (OVA) in different structural states and the allergic response triggered after intragastric or food challenges were assessed. BALB/c mice were sensitized intraperitoneally to OVA (50 mu g) in different structural states (native OVA, N-OVA; denatured OVA, D-OVA; formaldehyde- and lysine-treated OVA, FK-OVA; denatured OVA-FK, OVA-DFK; peptides from pepsin digestion, Pep-OVA). Anti-OVA-specific IgE responses were evaluated using ELISA. Anaphylactic signs and mMCP-1 serum levels were evaluated after intragastric (2.0 mg/OVA) and food (0.41 mg/OVA) challenges. IgE reactivities to N-OVA and D-OVA were similar among groups (p > 0.05). After the challenges, all OVA-sensitized mice developed mild to severe anaphylactic signs (p < 0.05 vs. control). Mice sensitized to N-OVA and D-OVA had the highest mMCP-1 serum levels after challenges (p < 0.05 vs. control). Allergic responses were similar despite the different OVA doses used for the challenges. The N-OVA-sensitized murine model of egg allergy proposed in the present study holds the potential for evaluating the impact of food matrix composition and processing on the threshold of egg-allergic responses.
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