期刊
CHEMOSENSORS
卷 11, 期 8, 页码 -出版社
MDPI
DOI: 10.3390/chemosensors11080425
关键词
near-infrared spectroscopy; water structure; extra-cellular pH; intra-cellular pH; partial least squares regression
In this study, the characteristic absorption of water in the near-infrared (NIR) region was utilized to determine extra-cellular pH (pHe) and intra-cellular pH (pHi). The use of continuous wavelet transform (CWT) enhanced the resolution of the spectra and partial least squares (PLS) regression was employed to establish quantitative models for pHe and pHi. The results showed that water with different hydrogen bonds can serve as a good probe to sense pH within biological systems.
Accurate determination of extra-cellular pH (pHe) and intra-cellular pH (pHi) is important to cancer diagnosis and treatment because tumor cells exhibit a lower pHe and a slightly higher pHi than normal cells. In this work, the characteristic absorption of water in the near-infrared (NIR) region was utilized for the determination of pHe and pHi. Dulbecco's modified eagle medium (DMEM) and bis (2-ethylhexyl) succinate sodium sulfonate reverse micelles (RM) were employed to simulate the extra- and intra-cellular fluids, respectively. Continuous wavelet transform (CWT) was used to enhance the resolution of the spectra. Quantitative models for pHe and pHi were established using partial least squares (PLS) regression, producing relative errors of validation samples in a range of -0.74-2.07% and -1.40-0.83%, respectively. Variable selection was performed, and the correspondence between the selected wavenumbers and water structures was obtained. Therefore, water with different hydrogen bonds may serve as a good probe to sense pH within biological systems.
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