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Article
Chemistry, Analytical
Yunlong Fan et al.
Summary: This study proposes a fast, highly automated, and low-consumption nucleic acid extraction method. By analyzing the process of magnetic beads crossing the gas-liquid interface, large magnetic bead packages were driven to cross the interface using a low magnetic field strength. This method provides a solution for high magnetic bead recovery rate in solid-phase extraction with a low-surfactant system based on a gas-liquid immiscible phase valve. The recovery rate of magnetic beads was improved to 90%-95%, and the carryover of reagents was below 1%.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Agriculture, Dairy & Animal Science
Paul Dawson et al.
Summary: The combination of PCR and DNA extraction techniques can be used to enumerate Campylobacter spp. from poultry gastrointestinal tract samples. The study developed a DNA standard curve for Campylobacter spp. DNA primers and a cell/genomic DNA extraction protocol for complex samples. The results showed that qPCR and standard plate count methods were highly correlated for enumerating Campylobacter spp. in the specified range.
Article
Chemistry, Analytical
Xiuen Cao et al.
Summary: This review summarizes the applications of functional nucleic acids and isothermal amplification techniques in biosensing, highlighting their important contributions to the field. These technologies have great potential to enhance the performance of biosensors.
Article
Chemistry, Analytical
Le Yu et al.
Summary: The gold standard for COVID-19 diagnosis is based on RT-PCR using centralized facilities and commercial viral RNA extraction kits. Due to shortages at the beginning of the pandemic, alternative low-cost magnetic nanoparticles (MNPs) were developed from biowaste for viral RNA extraction. These MNPs, with their unique nanoplatelet shape and high surface area, showed comparable performance to commercial magnetic beads in high throughput PCR analysis and could significantly reduce the cost of nucleic acid extraction.
Article
Spectroscopy
Mubashir Hussain et al.
Summary: This study proposes a novel pathogen detection system based on cultivating microdroplets and acquiring scattered light signals using a microfluidic device. The microdroplets are initially generated and incubated to cultivate bacteria. Then, the incubated droplets are reinjected into the detection module and passed through laser light to acquire the scattered light signals. The features obtained are trained using machine learning classifiers, and the k-nearest neighbors algorithm demonstrates superior classification performance with 95.6% accuracy for identifying P. aeruginosa.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Chemistry, Multidisciplinary
Jieyu Liu et al.
Summary: Compared with other types of breast cancer, triple-negative breast cancer (TNBC) has a high degree of malignancy and poor prognosis. Early diagnosis of TNBC through specific miRNA expression profiles can reduce its mortality. In this study, we developed a high-throughput, high-sensitivity method for quantitatively detecting multiple sets of TNBC-associated miRNAs using rolling circle amplification on fluorescence-encoded microspheres. The method showed extensive linear dynamic range, high sensitivity, reasonable specificity, and good recovery and reproducibility in serum.
CHINESE CHEMICAL LETTERS
(2023)
Article
Chemistry, Multidisciplinary
Yile Fang et al.
Summary: In this paper, a self-contained cartridge with a simple structure was developed for point-of-care detection of infectious diseases. The cartridge utilizes magnetic nanoparticles for nucleic acid extraction and automated detection process. The performance tests showed that it met the demands of automated nucleic acid detection, and it successfully detected hepatitis B virus and severe acute respiratory syndrome coronavirus 2.
CHINESE CHEMICAL LETTERS
(2023)
Article
Medicine, Research & Experimental
Yuan Guo et al.
Summary: This study investigated the effects of the cholinesterase inhibitor donepezil on lung fibrosis-induced pulmonary hypertension in a mouse model. The results showed that donepezil improved pulmonary artery and right ventricle remodeling, inhibited lung fibrosis and inflammation, and regulated Th17 cell differentiation.
Review
Biochemical Research Methods
Yuan Yuan Zhang et al.
Summary: This review provides an update on the limit of detection of electrochemical nucleic acid biosensors and classifies them based on the nature of the electrochemical transduction pathway. It offers an overall picture of the evolution of detection limits in various sensor architectures developed over the last three decades, along with a critical report on recent strategies.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Alexander Ecke et al.
Summary: Increasing contamination of environmental waters with pharmaceuticals requires fast and reliable analytical methods. The magnetic bead-based immunoassay reported here for the determination of diclofenac in water samples offers a significantly lower limit of detection than traditional ELISA, with shorter operating time and simplicity.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Zhu Chen et al.
Summary: The newly developed device shows high sensitivity and good specificity for Enterocytozoon hepatopenaei infection, enhancing stability, uniformity, and shortening testing time, with efficient light absorbance capability.
CHINESE CHEMICAL LETTERS
(2022)
Article
Chemistry, Multidisciplinary
Yingjia Liu et al.
Summary: A microfluidic biosensor based on immunomagnetic separation, enzyme catalysis, and electrochemical impedance analysis was developed for rapid and sensitive detection of S. typhimurium. The biosensor could quantitatively detect S. typhimurium in 1 hour and demonstrated good feasibility for practical applications.
CHINESE CHEMICAL LETTERS
(2022)
Article
Chemistry, Multidisciplinary
Sha Liu et al.
Summary: Listeriosis, caused by Listeria monocytogenes (LM), is a major global foodborne disease with a high mortality rate of 20% to 30%. Current LM detection methods are time-consuming and have low sensitivity. This study developed a rapid detection method using SYTO9 staining and image processing, based on loop-mediated isothermal amplification (LAMP). The method was simple, sensitive, and rapid, providing a new approach for the rapid detection of LM and other foodborne bacterial pathogens.
CHINESE CHEMICAL LETTERS
(2022)
Article
Biochemical Research Methods
Simon Haile et al.
Summary: The COVID-19 pandemic has shown the importance of generic reagents and flexible systems in diagnostic testing. Magnetic bead-based nucleic acid extraction protocols using 96-well plates on open liquid handlers have been optimized to reduce cross-well contamination and maintain sensitivity.
JOURNAL OF VIROLOGICAL METHODS
(2022)
Article
Chemistry, Analytical
Jiawei Hong et al.
Summary: An ionic liquid-based dispersive liquid-liquid microextraction combined with magnetic solid-phase extraction method was developed for extraction of quinolones from honey and milk. Satisfactory linearity and high sensitivity were achieved under optimized conditions. The proposed method can be employed for the determination of antibiotic residues in honey and milk samples.
Article
Biophysics
Huynh Quoc Nguyen et al.
Summary: The urgent need for rapid and accurate testing tools for SARS-CoV-2 detection can be met by IoT-based diagnostic devices, which can provide real-time monitoring with high sensitivity and accuracy.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Multidisciplinary
Gaowa Xing et al.
Summary: This article discusses the advances in bacteria detection using microfluidic biosensors. It introduces typical methods for pathogenic bacteria capture, separation, and detection, as well as key applications of microfluidic biosensor-based rapid bacteria detection. Furthermore, it concludes and discusses possible research prospects in the field of microfluidic biosensors for the rapid detection of pathogenic bacteria.
CHINESE CHEMICAL LETTERS
(2022)
Article
Chemistry, Analytical
Xiaoyu Zhao et al.
Summary: Researchers have developed a centrifugal microfluidic chip integrated with chitosan-modified magnetic microspheres for pH-induced nucleic acid extraction. The system demonstrates comparable performance in terms of nucleic acid concentration and purity, detection specificity and stability, and fast detection duration. This development is significant for portable, efficient, and simple nucleic acid diagnosis, especially under resource-limited conditions.
Article
Thermodynamics
K. K. Athira et al.
Summary: Aqueous biphasic system (ABS) is an efficient separation process that can be used for the extraction and purification of DNA. Ammonium-based ionic liquids (ILs) play an important role in ABS, and their structures have an influence on phase formation. By carefully selecting the components of ABS, a biocompatible system with controlled pH can be obtained for the partitioning of biomolecules. Experimental results showed that ABS containing benzyltributylammoniumchloride ([BTBA]Cl) IL exhibited high efficiency in DNA partitioning.
FLUID PHASE EQUILIBRIA
(2022)
Article
Biotechnology & Applied Microbiology
Rachel L. Byrne et al.
Summary: The study aims to develop a low-cost method for isolating ARGs from river water. The MagnaExtract method was found to be comparable, and in some cases superior to commercially available kits for this purpose. This simple and high-yield extraction method could be beneficial for surveillance campaigns in East Africa.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Chemistry, Inorganic & Nuclear
Anastasia Bulgakova et al.
Summary: Magnetic Fe3O4 nanoparticles have been widely studied for their potential applications in nucleic acid separation, detection, and delivery. In this study, magnetic nylon 6 nanocomposites were synthesized, and a novel method of UV-induced immobilization of oligonucleotides on these nanocomposites for nucleic acid magnetic separation was proposed. The results showed that the synthesized nanocomposites exhibited high oligonucleotide binding capacity and loading efficiency, making them attractive candidates for nucleic acid-specific magnetic isolation.
Article
Parasitology
Jenny Knapp et al.
Summary: Confirmed diagnosis of alveolar echinococcosis (AE) relies on pathological criteria and molecular evidence. This study evaluated various PCR techniques for the diagnosis of AE in patients and identified the most effective strategy for molecular diagnosis based on the nature of the tested samples. The results showed that a qPCR assay targeting mitochondrial genes exhibited higher sensitivity in detecting AE samples.
Review
Chemistry, Multidisciplinary
Lei He et al.
Summary: Aptamers have high specificity and affinity toward various targets, making them widely used in analytical techniques. In order to improve biosensor sensitivity, different amplification strategies are being explored, such as using nanomaterials or enzyme mimics.
CHINESE CHEMICAL LETTERS
(2021)
Article
Biochemical Research Methods
Benjamin P. Sullivan et al.
Summary: The translation introduces a paper-based device that uses isotachophoresis to extract and purify nucleic acids from whole blood, providing a simpler sample preparation method for point-of-care NAATs.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Review
Medicine, General & Internal
Choo Yee Yu et al.
Summary: COVID-19, originating from Wuhan, China, has spread globally with devastating impacts on society, economy, and healthcare systems. Accurate and rapid diagnosis remains crucial, with authorized nucleic acid-based tests serving as alternatives.
Review
Chemistry, Analytical
Daniele Obino et al.
Summary: Nucleic acid extraction is a fundamental process for genetic analysis, involving steps such as cell separation, cell lysis, and nucleic acid isolation. Microfluidic devices have been developed to enable efficient analysis of nucleic acid samples.
Article
Microbiology
Tao Zuo et al.
Summary: Patients with COVID-19 exhibited distinctive gut virome composition compared to non-COVID-19 subjects, which persisted even after the resolution of the disease. The findings suggest that the gut virome may play a role in modulating host immunity and impacting the severity of SARS-CoV-2 infection.
Letter
Medical Laboratory Technology
Si-Yu Chen et al.
Summary: The study found good agreement between the Maelstrom(TM) 8 and QIAamp Viral RNA Mini Kit in nucleic acid extraction, with a mean cycle threshold value of 29.1. The Maelstrom(TM) 8 offers standardized procedures, avoidance of cross contaminations, ease of use, faster turnaround time, and less hands-on time compared to manual extraction methods, making it more suitable for clinical laboratories with small to medium-sized sample volumes.
CLINICAL LABORATORY
(2021)
Review
Materials Science, Multidisciplinary
Xuehui Xu et al.
Summary: Nucleic acid logic devices are highly flexible and responsive, able to perform logic operations in complex biological environments. They can respond to various stimuli, such as DNA/RNA strands, metal ions, and small molecules.
Article
Biochemical Research Methods
David J. Clark et al.
Article
Nanoscience & Nanotechnology
Stephanie Pearlman et al.
ACS APPLIED MATERIALS & INTERFACES
(2020)
Review
Chemistry, Applied
Rui Zhang et al.
CARBOHYDRATE POLYMERS
(2020)
Article
Biochemistry & Molecular Biology
Linlin Hou et al.
NUCLEIC ACIDS RESEARCH
(2020)
Review
Chemistry, Analytical
Maryam Arabi et al.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2020)
Article
Chemistry, Physical
Shixiang Liu et al.
ADVANCES IN COLLOID AND INTERFACE SCIENCE
(2020)
Review
Chemistry, Analytical
Yanju Chen et al.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2020)
Review
Chemistry, Analytical
Miranda N. Emaus et al.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2020)
Article
Chemistry, Analytical
Karla Perez-Toralla et al.
SENSORS AND ACTUATORS B-CHEMICAL
(2019)
Review
Biochemistry & Molecular Biology
Muhammad Faisal Manzoor et al.
JOURNAL OF FOOD BIOCHEMISTRY
(2019)
Article
Chemistry, Multidisciplinary
Zeeshan Ali et al.
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
(2017)
Article
Multidisciplinary Sciences
Palak G. Patel et al.
Review
Biotechnology & Applied Microbiology
Adilah Ayoib et al.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2017)
Article
Chemistry, Analytical
Akemi Yamaguchi et al.
ANALYTICA CHIMICA ACTA
(2016)
Article
Microbiology
Hagen Frickmann et al.
EUROPEAN JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
(2015)
Article
Pathology
Nicola Dundas et al.
JOURNAL OF MOLECULAR DIAGNOSTICS
(2008)
Article
Biotechnology & Applied Microbiology
Hiroyuki Ota et al.
JOURNAL OF BIOTECHNOLOGY
(2006)
