期刊
BIOSENSORS-BASEL
卷 13, 期 7, 页码 -出版社
MDPI
DOI: 10.3390/bios13070753
关键词
smartphone; fluorescence; glass capillary; biomarkers; RGB
The sensitive and rapid detection of microsamples is crucial for early diagnosis of diseases. We developed a low-cost smartphone-based fluorescence detection device (Smartphone-BFDD) without precision equipment for rapid identification and quantification of biomarkers on glass capillary. The device combines microfluidic technology with RGB image analysis, effectively reducing the sample volume to 20 μL and detection time to only 30 min. This device provides potential applications for different biomarkers and offers wide use for rapid biochemical analysis in biomedical research.
The sensitive and rapid detection of microsamples is crucial for early diagnosis of diseases. The short response times and low sample volume requirements of microfluidic chips have shown great potential in early diagnosis, but there are still shortcomings such as complex preparation processes and high costs. We developed a low-cost smartphone-based fluorescence detection device (Smartphone-BFDD) without precision equipment for rapid identification and quantification of biomarkers on glass capillary. The device combines microfluidic technology with RGB image analysis, effectively reducing the sample volume to 20 & mu;L and detection time to only 30 min. For the sensitivity of the device, we constructed a standard sandwich immunoassay (antibody-antigen-antibody) in a glass capillary using the N-protein of SARS-CoV-2 as a biological model, realizing a low limit of detection (LOD, 40 ng mL(-1)). This device provides potential applications for different biomarkers and offers wide use for rapid biochemical analysis in biomedical research.
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