相关参考文献
注意:仅列出部分参考文献,下载原文获取全部文献信息。Potential pitfalls of CRISPR/Cas9-mediated genome editing
Rongxue Peng et al.
FEBS JOURNAL (2016)
Efficient identification of CRISPR/Cas9-induced insertions/deletions by direct germline screening in zebrafish
Isabel Brocal et al.
BMC GENOMICS (2016)
Maximizing mutagenesis with solubilized CRISPR-Cas9 ribonucleoprotein complexes
Alexa Burger et al.
DEVELOPMENT (2016)
Regulated protein depletion by the auxin-inducible degradation system in Drosophila melanogaster
Martina Trost et al.
FLY (2016)
The application of somatic CRISPR-Cas9 to conditional genome editing in Caenorhabditis elegans
Wei Li et al.
GENESIS (2016)
Efficient introduction of specific homozygous and heterozygous mutations using CRISPR/Cas9
Dominik Paquet et al.
NATURE (2016)
Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage
Alexis C. Komor et al.
NATURE (2016)
CRISPR knockout screening outperforms shRNA and CRISPRi in identifying essential genes
Bastiaan Evers et al.
NATURE BIOTECHNOLOGY (2016)
Systematic comparison of CRISPR/Cas9 and RNAi screens for essential genes
David W. Morgens et al.
NATURE BIOTECHNOLOGY (2016)
Organoids as an in vitro model of human development and disease
Aliya Fatehullah et al.
NATURE CELL BIOLOGY (2016)
Parallel shRNA and CRISPR-Cas9 screens enable antiviral drug target identification
Richard M. Deans et al.
NATURE CHEMICAL BIOLOGY (2016)
Editing the epigenome: technologies for programmable transcription and epigenetic modulation
Pratiksha I. Thakore et al.
NATURE METHODS (2016)
Comparison of Cas9 activators in multiple species
Alejandro Chavez et al.
NATURE METHODS (2016)
Defining and improving the genome-wide specificities of CRISPR-Cas9 nucleases
Shengdar Q. Tsai et al.
NATURE REVIEWS GENETICS (2016)
DIANA-mirExTra v2.0: Uncovering microRNAs and transcription factors with crucial roles in NGS expression data
Ioannis S. Vlachos et al.
NUCLEIC ACIDS RESEARCH (2016)
Effective knockdown of Drosophila long non-coding RNAs by CRISPR interference
Sanjay Ghosh et al.
NUCLEIC ACIDS RESEARCH (2016)
Repurposing the CRISPR-Cas9 system for targeted DNA methylation
Aleksandar Vojta et al.
NUCLEIC ACIDS RESEARCH (2016)
Versatile in vivo regulation of tumor phenotypes by dCas9-mediated transcriptional perturbation
Christian J. Braun et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2016)
Reverse Genetics in Zebrafish: Mutants, Morphants, and Moving Forward
Nathan D. Lawson
TRENDS IN CELL BIOLOGY (2016)
CRISPR Screens Provide a Comprehensive Assessment of Cancer Vulnerabilities but Generate False-Positive Hits for Highly Amplified Genomic Regions
Diana M. Munoz et al.
CANCER DISCOVERY (2016)
Genomic Copy Number Dictates a Gene-Independent Cell Response to CRISPR/Cas9 Targeting
Andrew J. Aguirre et al.
CANCER DISCOVERY (2016)
Rapid Protein Depletion in Human Cells by Auxin-Inducible Degron Tagging with Short Homology Donors
Toyoaki Natsume et al.
CELL REPORTS (2016)
A genome-wide resource for the analysis of protein localisation in Drosophila
Mihail Sarov et al.
ELIFE (2016)
Towards a compendium of essential genes - From model organisms to synthetic lethality in cancer cells
Tianzuo Zhan et al.
CRITICAL REVIEWS IN BIOCHEMISTRY AND MOLECULAR BIOLOGY (2016)
Rapid identification and recovery of ENU-induced mutations with next-generation sequencing and Paired-End Low-Error analysis
Luyuan Pan et al.
BMC GENOMICS (2015)
Apc Restoration Promotes Cellular Differentiation and Reestablishes Crypt Homeostasis in Colorectal Cancer
Lukas E. Dow et al.
CELL (2015)
Genome-wide CRISPR Screen in a Mouse Model of Tumor Growth and Metastasis
Sidi Chen et al.
CELL (2015)
A Genome-wide CRISPR Screen in Primary Immune Cells to Dissect Regulatory Networks
Oren Parnas et al.
CELL (2015)
Regulation of transcriptionally active genes via the catalytically inactive Cas9 in C-elegans and D-rerio
Lijiang Long et al.
CELL RESEARCH (2015)
A photosensitive degron enables acute light-induced protein degradation in the nervous system
Anke Hermann et al.
CURRENT BIOLOGY (2015)
Anillin Regulates Neuronal Migration and Neurite Growth by Linking RhoG to the Actin Cytoskeleton
Dong Tian et al.
CURRENT BIOLOGY (2015)
The auxin-inducible degradation (AID) system enables versatile conditional protein depletion in C-elegans
Liangyu Zhang et al.
DEVELOPMENT (2015)
Making Sense of Anti-Sense Data
Didier Y. R. Stainier et al.
DEVELOPMENTAL CELL (2015)
Morpholinos: Antisense and Sensibility
Martin Blum et al.
DEVELOPMENTAL CELL (2015)
A CRISPR/Cas9 Vector System for Tissue-Specific Gene Disruption in Zebrafish
Julien Ablain et al.
DEVELOPMENTAL CELL (2015)
Reverse Genetic Screening Reveals Poor Correlation between Morpholino-Induced and Mutant Phenotypes in Zebrafish
Fatma O. Kok et al.
DEVELOPMENTAL CELL (2015)
Genetic screens and functional genomics using CRISPR/Cas9 technology
Ella Hartenian et al.
FEBS JOURNAL (2015)
A Transparent Window into Biology: A Primer on Caenorhabditis elegans
Ann K. Corsi et al.
GENETICS (2015)
In Vivo Transcriptional Activation Using CRISPR/Cas9 in Drosophila
Shuailiang Lin et al.
GENETICS (2015)
High Efficiency, Homology-Directed Genome Editing in Caenorhabditis elegans Using CRISPR-Cas9 Ribonucleoprotein Complexes
Alexandre Paix et al.
GENETICS (2015)
Enabling functional genomics with genome engineering
Isaac B. Hilton et al.
GENOME RESEARCH (2015)
Somatic CRISPR-Cas9-induced mutations reveal roles of embryonically essential dynein chains in Caenorhabditis elegans cilia
Wenjing Li et al.
JOURNAL OF CELL BIOLOGY (2015)
Big Data in Caenorhabditis elegans: quo vadis?
Harald Hutter et al.
MOLECULAR BIOLOGY OF THE CELL (2015)
Global genetic analysis in mice unveils central role for cilia in congenital heart disease
You Li et al.
NATURE (2015)
Genetic compensation induced by deleterious mutations but not gene knockdowns
Andrea Rossi et al.
NATURE (2015)
Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex
Silvana Konermann et al.
NATURE (2015)
Inducible in vivo genome editing with CRISPR-Cas9
Lukas E. Dow et al.
NATURE BIOTECHNOLOGY (2015)
Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers
Isaac B. Hilton et al.
NATURE BIOTECHNOLOGY (2015)
The promise and peril of chemical probes
Cheryl H. Arrowsmith et al.
NATURE CHEMICAL BIOLOGY (2015)
Functional annotation of native enhancers with a Cas9-histone demethylase fusion
Nicola A. Kearns et al.
NATURE METHODS (2015)
Highly efficient Cas9-mediated transcriptional programming
Alejandro Chavez et al.
NATURE METHODS (2015)
Highly specific epigenome editing by CRISPR-Cas9 repressors for silencing of distal regulatory elements
Pratiksha I. Thakore et al.
NATURE METHODS (2015)
High-throughput functional genomics using CRISPR-Cas9
Ophir Shalem et al.
NATURE REVIEWS GENETICS (2015)
Next-generation libraries for robust RNA interference-based genome-wide screens
Martin Kampmann et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2015)
Auxin binding protein 1 (ABP1) is not required for either auxin signaling or Arabidopsis development
Yangbin Gao et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2015)
Gene essentiality and synthetic lethality in haploid human cells
Vincent A. Blomen et al.
SCIENCE (2015)
Identification and characterization of essential genes in the human genome
Tim Wang et al.
SCIENCE (2015)
Identification of potential drug targets for tuberous sclerosis complex by synthetic screens combining CRISPR-based knockouts with RNAi
Benjamin E. Housden et al.
SCIENCE SIGNALING (2015)
Quantifying on- and off-target genome editing
Ayal Hendel et al.
TRENDS IN BIOTECHNOLOGY (2015)
RIPK1 and RIPK3: critical regulators of inflammation and cell death
Kim Newton
TRENDS IN CELL BIOLOGY (2015)
Optogenetic mutagenesis in Caenorhabditis elegans
Kentaro Noma et al.
NATURE COMMUNICATIONS (2015)
Recombinase-based conditional and reversible gene regulation via XTR alleles
Camila Robles-Oteiza et al.
NATURE COMMUNICATIONS (2015)
Nanobody-targeted E3-ubiquitin ligase complex degrades nuclear proteins
Yeong Ju Shin et al.
SCIENTIFIC REPORTS (2015)
Probing Yeast Polarity with Acute, Reversible, Optogenetic Inhibition of Protein Function
Anna Payne-Tobin Jost et al.
ACS SYNTHETIC BIOLOGY (2015)
A Multifunctional Mutagenesis System for Analysis of Gene Function in Zebrafish
Helen Ngoc Bao Quach et al.
G3-GENES GENOMES GENETICS (2015)
A Transparent Window into Biology: A Primer on Caenorhabditis elegans
Ann K. Corsi et al.
GENETICS (2015)
In Vivo Transcriptional Activation Using CRISPR/Cas9 in Drosophila
Shuailiang Lin et al.
GENETICS (2015)
Identification of potential drug targets for tuberous sclerosis complex by synthetic screens combining CRISPR-based knockouts with RNAi
Benjamin E. Housden et al.
SCIENCE SIGNALING (2015)
klf2ash317 Mutant Zebrafish Do Not Recapitulate Morpholino-Induced Vascular and Haematopoietic Phenotypes
Peter Novodvorsky et al.
PLOS ONE (2015)
CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling
Randall J. Platt et al.
CELL (2014)
A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging
Marvin E. Tanenbaum et al.
CELL (2014)
Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation
Luke A. Gilbert et al.
CELL (2014)
Chemical Biology Strategies for Posttranslational Control of Protein Function
Rishi Rakhit et al.
CHEMISTRY & BIOLOGY (2014)
Repurposing an endogenous degradation system for rapid and targeted depletion of C. elegans proteins
Stephen T. Armenti et al.
DEVELOPMENT (2014)
Analysis of the expression patterns, subcellular localisations and interaction partners of Drosophila proteins using a pigP protein trap library
Nick Lowe et al.
DEVELOPMENT (2014)
Out with the old, in with the new: reassessing morpholino knockdowns in light of genome editing technology
Stefan Schulte-Merker et al.
DEVELOPMENT (2014)
High-Resolution Temporal Analysis Reveals a Functional Timeline for the Molecular Regulation of Cytokinesis
Tim Davies et al.
DEVELOPMENTAL CELL (2014)
Conditional Knockouts Generated by Engineered CRISPR-Cas9 Endonuclease Reveal the Roles of Coronin in C. elegans Neural Development
Zhongfu Shen et al.
DEVELOPMENTAL CELL (2014)
Resources for Functional Genomics Studies in Drosophila melanogaster
Stephanie E. Mohr et al.
GENETICS (2014)
Scalable and Versatile Genome Editing Using Linear DNAs with Microhomology to Cas9 Sites in Caenorhabditis elegans
Alexandre Paix et al.
GENETICS (2014)
Efficient Marker-Free Recovery of Custom Genetic Modifications with CRISPR/Cas9 in Caenorhabditis elegans
Joshua A. Arribere et al.
GENETICS (2014)
Megabase-scale deletion using CRISPR/Cas9 to generate a fully haploid human cell line
Patrick Essletzbichler et al.
GENOME RESEARCH (2014)
Stable RNA interference rules for silencing
Christof Fellmann et al.
NATURE CELL BIOLOGY (2014)
RNAi screening comes of age: improved techniques and complementary approaches
Stephanie E. Mohr et al.
NATURE REVIEWS MOLECULAR CELL BIOLOGY (2014)
Chemical mutagens, transposons, and transgenes to interrogate gene function in Drosophila melanogaster
Koen J. T. Venken et al.
METHODS (2014)
RNAi screening in Drosophila cells and in vivo
Stephanie E. Mohr
METHODS (2014)
The Serine-Threonine Protein Kinase PAK4 Is Dispensable in Zebrafish: Identification of a Morpholino-Generated Pseudophenotype
Sheran H. W. Law et al.
PLOS ONE (2014)
Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells
Ophir Shalem et al.
SCIENCE (2014)
Genetic Screens in Human Cells Using the CRISPR-Cas9 System
Tim Wang et al.
SCIENCE (2014)
CRISPR/Cas9 Mediates Efficient Conditional Mutagenesis in Drosophila
Zhaoyu Xue et al.
G3-GENES GENOMES GENETICS (2014)
Mutagenesis and homologous recombination in Drosophila cell lines using CRISPR/Cas9
Andrew R. Bassett et al.
BIOLOGY OPEN (2014)
Parallel genome-scale loss of function screens in 216 cancer cell lines for the identification of context-specific genetic dependencies
Glenn S. Cowley et al.
SCIENTIFIC DATA (2014)
One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering
Haoyi Wang et al.
CELL (2013)
One-Step Generation of Mice Carrying Reporter and Conditional Alleles by CRISPR/Cas-Mediated Genome Engineering
Hui Yang et al.
CELL (2013)
A Systematic Mammalian Genetic Interaction Map Reveals Pathways Underlying Ricin Susceptibility
Michael C. Bassik et al.
CELL (2013)
Beyond Secondary Structure: Primary-Sequence Determinants License Pri-miRNA Hairpins for Processing
Vincent C. Auyeung et al.
CELL (2013)
CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes
Luke A. Gilbert et al.
CELL (2013)
Genome editing with RNA-guided Cas9 nuclease in Zebrafish embryos
Nannan Chang et al.
CELL RESEARCH (2013)
A combinatorial regulatory signature controls terminal differentiation of the dopaminergic nervous system in C. elegans
Maria Doitsidou et al.
GENES & DEVELOPMENT (2013)
Genome Engineering of Drosophila with the CRISPR RNA-Guided Cas9 Nuclease
Scott J. Gratz et al.
GENETICS (2013)
A large-scale zebrafish gene knockout resource for the genome-wide study of gene function
Gaurav K. Varshney et al.
GENOME RESEARCH (2013)
The million mutation project: A new approach to genetics in Caenorhabditis elegans
Owen Thompson et al.
GENOME RESEARCH (2013)
Genome-wide Consequences of Deleting Any Single Gene
Xinchen Teng et al.
MOLECULAR CELL (2013)
A systematic genome-wide analysis of zebrafish protein-coding gene function
Ross N. W. Kettleborough et al.
NATURE (2013)
CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering
Prashant Mali et al.
NATURE BIOTECHNOLOGY (2013)
Efficient genome editing in zebrafish using a CRISPR-Cas system
Woong Y. Hwang et al.
NATURE BIOTECHNOLOGY (2013)
Engineering the Caenorhabditis elegans genome using Cas9-triggered homologous recombination
Daniel J. Dickinson et al.
NATURE METHODS (2013)
RNA-guided gene activation by CRISPR-Cas9-based transcription factors
Pablo Perez-Pinera et al.
NATURE METHODS (2013)
CRISPR RNA-guided activation of endogenous human genes
Morgan L. Maeder et al.
NATURE METHODS (2013)
A reversible gene trap collection empowers haploid genetics in human cells
Tilmann Buerckstuemmer et al.
NATURE METHODS (2013)
Heritable genome editing in C. elegans via a CRISPR-Cas9 system
Ari E. Friedland et al.
NATURE METHODS (2013)
Treatment of HCV Infection by Targeting MicroRNA
Harry L. A. Janssen et al.
NEW ENGLAND JOURNAL OF MEDICINE (2013)
RNA-Guided Human Genome Engineering via Cas9
Prashant Mali et al.
SCIENCE (2013)
Multiplex Genome Engineering Using CRISPR/Cas Systems
Le Cong et al.
SCIENCE (2013)
Conserved Regulators of Nucleolar Size Revealed by Global Phenotypic Analyses
Ralph A. Neumueller et al.
SCIENCE SIGNALING (2013)
ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering
Thomas Gaj et al.
TRENDS IN BIOTECHNOLOGY (2013)
PAPTi: A Peptide Aptamer Interference Toolkit for Perturbation of Protein-Protein Interaction Networks
Johannes T. -H. Yeh et al.
SCIENTIFIC REPORTS (2013)
An Optimized microRNA Backbone for Effective Single-Copy RNAi
Christof Fellmann et al.
CELL REPORTS (2013)
A simple strategy for heritable chromosomal deletions in zebrafish via the combinatorial action of targeting nucleases
Shimin Lim et al.
GENOME BIOLOGY (2013)
The Loop Position of shRNAs and Pre-miRNAs Is Critical for the Accuracy of Dicer Processing In Vivo
Shuo Gu et al.
CELL (2012)
Generation of Genetically Modified Mice by Oocyte Injection of Androgenetic Haploid Embryonic Stem Cells
Hui Yang et al.
CELL (2012)
Essential metabolic, anti-inflammatory, and anti-tumorigenic functions of miR-122 in liver
Shu-hao Hsu et al.
JOURNAL OF CLINICAL INVESTIGATION (2012)
MicroRNA-122 plays a critical role in liver homeostasis and hepatocarcinogenesis
Wei-Chih Tsai et al.
JOURNAL OF CLINICAL INVESTIGATION (2012)
Fluorescent fusion protein knockout mediated by anti-GFP nanobody
Emmanuel Caussinus et al.
NATURE STRUCTURAL & MOLECULAR BIOLOGY (2012)
A Genome-Wide Collection of Mos1 Transposon Insertion Mutants for the C. elegans Research Community
Elodie Vallin et al.
PLOS ONE (2012)
Knockout of the prion protein (PrP)-like Sprn gene does not produce embryonic lethality in combination with PrPC-deficiency
Nathalie Daude et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2012)
Inducible, reversible system for the rapid and complete degradation of proteins in mammalian cells
Andrew J. Holland et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2012)
Essential Gene Profiles in Breast, Pancreatic, and Ovarian Cancer Cells
Richard Marcotte et al.
CANCER DISCOVERY (2012)
Large-Scale Screening for Targeted Knockouts in the Caenorhabditis elegans Genome
Robert Barstead et al.
G3-GENES GENOMES GENETICS (2012)
The use of RNAi technologies for gene knockdown in zebrafish
Amanda Kelly et al.
BRIEFINGS IN FUNCTIONAL GENOMICS (2011)
A Rapid and Scalable System for Studying Gene Function in Mice Using Conditional RNA Interference
Prem K. Premsrirut et al.
CELL (2011)
Synthetic lethality: General principles, utility and detection using genetic screens in human cells
Sebastian M. B. Nijman
FEBS LETTERS (2011)
A versatile gene trap to visualize and interrogate the function of the vertebrate proteome
Le A. Trinh et al.
GENES & DEVELOPMENT (2011)
Functional Identification of Optimized RNAi Triggers Using a Massively Parallel Sensor Assay
Christof Fellmann et al.
MOLECULAR CELL (2011)
Derivation of haploid embryonic stem cells from mouse embryos
Martin Leeb et al.
NATURE (2011)
Toolkit for evaluating genes required for proliferation and survival using tetracycline-regulated RNAi
Johannes Zuber et al.
NATURE BIOTECHNOLOGY (2011)
Small-molecule displacement of a cryptic degron causes conditional protein degradation
Kimberly M. Bonger et al.
NATURE CHEMICAL BIOLOGY (2011)
Mapping of signaling networks through synthetic genetic interaction analysis by RNAi
Thomas Horn et al.
NATURE METHODS (2011)
Quantification of PtdInsP3 molecular species in cells and tissues by mass spectrometry
Jonathan Clark et al.
NATURE METHODS (2011)
MiMIC: a highly versatile transposon insertion resource for engineering Drosophila melanogaster genes
Koen J. T. Venken et al.
NATURE METHODS (2011)
A Survey of New Temperature-Sensitive, Embryonic-Lethal Mutations in C. elegans: 24 Alleles of Thirteen Genes
Sean M. O'Rourke et al.
PLOS ONE (2011)
Reversible suppression of an essential gene in adult mice using transgenic RNA interference
Katherine McJunkin et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2011)
Systematic investigation of genetic vulnerabilities across cancer cell lines reveals lineage-specific dependencies in ovarian cancer
Hiu Wing Cheung et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2011)
Analysis of Multiple Ethyl Methanesulfonate-Mutagenized Caenorhabditis elegans Strains by Whole-Genome Sequencing
Sumeet Sarin et al.
GENETICS (2010)
Targeted chromosomal deletions in human cells using zinc finger nucleases
Hyung Joo Lee et al.
GENOME RESEARCH (2010)
Autonomous role of medullary thymic epithelial cells in central CD4+ T cell tolerance
Maria Hinterberger et al.
NATURE IMMUNOLOGY (2010)
In vivo RNAi: Today and Tomorrow
Norbert Perrimon et al.
COLD SPRING HARBOR PERSPECTIVES IN BIOLOGY (2010)
An auxin-based degron system for the rapid depletion of proteins in nonplant cells
Kohei Nishimura et al.
NATURE METHODS (2009)
Control of alternative splicing through siRNA-mediated transcriptional gene silencing
Mariano Allo et al.
NATURE STRUCTURAL & MOLECULAR BIOLOGY (2009)
Haploid Genetic Screens in Human Cells Identify Host Factors Used by Pathogens
Jan E. Carette et al.
SCIENCE (2009)
Artificial miRNAs mitigate shRNA-mediated toxicity in the brain: Implications for the therapeutic development of RNAi
Jodi L. McBride et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2008)
Highly parallel identification of essential genes in cancer cells
Biao Luo et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2008)
Chromosomal passengers: conducting cell division
Sandrine Ruchaud et al.
NATURE REVIEWS MOLECULAR CELL BIOLOGY (2007)
A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila
Georg Dietzl et al.
NATURE (2007)
A transgene-assisted genetic screen identifies essential regulators of vascular development in vertebrate embryos
Suk-Won Jin et al.
DEVELOPMENTAL BIOLOGY (2007)
Tissue-specific and reversible RNA interference in transgenic mice
Ross A. Dickins et al.
NATURE GENETICS (2007)
Sleeping Beauty transposase has an affinity for heterochromatin conformation
Ryuji Ikeda et al.
MOLECULAR AND CELLULAR BIOLOGY (2007)
Morpholino, siRNA, and S-DNA compared: Impact of structure and mechanism of action on off-target effects and sequence specificity
James E. Summerton
CURRENT TOPICS IN MEDICINAL CHEMISTRY (2007)
Evidence of off-target effects associated with long dsRNAs in Drosophila melanogaster cell-based assays
Meghana M. Kulkarni et al.
NATURE METHODS (2006)
Prevalence of off-target effects in Drosophila RNA interference screens
Yong Ma et al.
NATURE (2006)
A rapid, reversible, and tunable method to regulate protein function in living cells using synthetic small molecules
Laura A. Banaszynski et al.
CELL (2006)
Transcriptional and phenotypic comparisons of Ppara knockout and siRNA knockdown mice
Angus T. De Souza et al.
NUCLEIC ACIDS RESEARCH (2006)
Validating Aurora B as an anti-cancer drug target
Fiona Girdler et al.
JOURNAL OF CELL SCIENCE (2006)
Fatality in mice due to oversaturation of cellular microRNA/short hairpin RNA pathways
Dirk Grimm et al.
NATURE (2006)
Polycistronic RNA polymerase II expression vectors for RNA interference based on BIC/miR-155
Kwan-Ho Chung et al.
NUCLEIC ACIDS RESEARCH (2006)
Probing tumor phenotypes using stable and regulated synthetic microRNA precursors
RA Dickins et al.
NATURE GENETICS (2005)
A lentiviral microRNA-based system for single-copy polymerase II-regulated RNA interference in mammalian cells
F Stegmeier et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2005)
Modulation of hepatitis C virus RNA abundance by a liver-specific microRNA
CL Jopling et al.
SCIENCE (2005)
Aurora kinases: shining lights on the therapeutic horizon?
PD Andrews
ONCOGENE (2005)
Cancer gene discovery in solid tumours using transposon-based somatic mutagenesis in the mouse
LS Collier et al.
NATURE (2005)
DNA transposons in vertebrate functional genomics
C Miskey et al.
CELLULAR AND MOLECULAR LIFE SCIENCES (2005)
The concept of synthetic lethality in the context of anticancer therapy
WG Kaelin
NATURE REVIEWS CANCER (2005)
Chemical genetic control of protein levels: Selective in vivo targeted degradation
JS Schneekloth et al.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2004)
A large-scale RNAi screen in human cells identifies new components of the p53 pathway
K Berns et al.
NATURE (2004)
A resource for large-scale RNA-interference-based screens in mammals
PJ Paddison et al.
NATURE (2004)
High-throughput RNAi in Caenorhabditis elegans:: genome-wide screens and functional genomics
A Sugimoto
DIFFERENTIATION (2004)
Temperature-sensitive control of protein activity by conditionally splicing inteins
MP Zeidler et al.
NATURE BIOTECHNOLOGY (2004)
Aurora-kinase inhibitors as anticancer agents
N Keen et al.
NATURE REVIEWS CANCER (2004)
A systematic genome-wide screen for mutations affecting organogenesis in Medaka, Oryzias latipes
M Furutani-Seiki et al.
MECHANISMS OF DEVELOPMENT (2004)
Mismatch repair genes identified using genetic screens in Blm-deficient embryonic stem cells
G Guo et al.
NATURE (2004)
A public gene trap resource for mouse functional genomics
WC Skarnes et al.
NATURE GENETICS (2004)
Caenorhabditis elegans lin-35/Rb, elf-1/E2F and other synthetic multivulva genes negatively regulate the anaphase-promoting complex gene mat-3/APC8
D Garbe et al.
GENETICS (2004)
The cellular geography of aurora kinases
M Carmena et al.
NATURE REVIEWS MOLECULAR CELL BIOLOGY (2003)
Acute mutation of retinoblastoma gene function is sufficient for cell cycle re-entry
J Sage et al.
NATURE (2003)
piggyBac-based insertional mutagenesis in the presence of stably integrated P elements in Drosophila
U Häcker et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2003)
Aurora B couples chromosome alignment with anaphase by targeting BubR1, Mad2, and Cenp-E to kinetochores
C Ditchfield et al.
JOURNAL OF CELL BIOLOGY (2003)
The small molecule Hesperadin reveals a role for Aurora B in correcting kinetochore-microtubule attachment and in maintaining the spindle assembly checkpoint
S Hauf et al.
JOURNAL OF CELL BIOLOGY (2003)
Systematic functional analysis of the Caenorhabditis elegans genome using RNAi
RS Kamath et al.
NATURE (2003)
Both natural and designed micro RNAs technique can inhibit the expression of cognate mRNAs when expressed in human cells
Y Zeng et al.
MOLECULAR CELL (2002)
Insertional mutagenesis in zebrafish rapidly identifies genes essential for early vertebrate development
G Golling et al.
NATURE GENETICS (2002)
A system for stable expression of short interfering RNAs in mammalian cells
TR Brummelkamp et al.
SCIENCE (2002)
The art and design of genetic screens: Drosophila melanogaster
D St Johnston
NATURE REVIEWS GENETICS (2002)
A protein trap strategy to detect GFP-tagged proteins expressed from their endogenous loci in Drosophila
X Morin et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2001)
Conditional control of gene expression in the mouse
M Lewandoski
NATURE REVIEWS GENETICS (2001)
Specific inhibition of gene expression by small double-stranded RNAs in invertebrate and vertebrate systems
NJ Caplen et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2001)
Protacs: Chimeric molecules that target proteins to the Skp1-Cullin-F box complex for ubiquitination and degradation
KM Sakamoto et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2001)
Efficient and heritable functional knock-out of an adult phenotype in Drosophila using a GAL4-driven hairpin RNA incorporating a heterologous spacer
Alberto Piccin et al.
NUCLEIC ACIDS RESEARCH (2001)
Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells
SM Elbashir et al.
NATURE (2001)
Effective targeted gene 'knockdown' in zebrafish
A Nasevicius et al.
NATURE GENETICS (2000)
A systematic, genome-wide, phenotype-driven mutagenesis programme for gene function studies in the mouse
PM Nolan et al.
NATURE GENETICS (2000)
Genetic requirements for inheritance of RNAi in C-elegans
A Grishok et al.
SCIENCE (2000)
Heritable and inducible genetic interference by double-stranded RNA encoded by transgenes
N Tavernarakis et al.
NATURE GENETICS (2000)