期刊
NATURE PROTOCOLS
卷 11, 期 3, 页码 456-475出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nprot.2016.018
关键词
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资金
- National Science Foundation
- National Defense Science and Engineering Graduate Fellowship
- US National Institutes of Health (NIH) [R01 CA186568]
- Howard Hughes Medical Institute Collaborative Initiative Award
This protocol describes a method to obtain spatially resolved proteomic maps of specific compartments within living mammalian cells. An engineered peroxidase, APEAPEAPEX2, is genetically targeted to a cellular region of interest. Upon the addition of hydrogen peroxide for 1 min to cells preloaded with a biotin-phenol substrate, APEAPEAPEX2 generates biotin-phenoxyl radicals that covalently tag proximal endogenous proteins. Cells are then lysed, and biotinylated proteins are enriched with streptavidin beads and identified by mass spectrometry. We describe the generation of an appropriate APEAPEAPEX2 fusion construct, proteomic sample preparation, and mass spectrometric data acquisition and analysis. A two-state stable isotope labeling by amino acids in cell culture (SILACLACLAC) protocol is used for proteomic mapping of membrane-enclosed cellular compartments from which APEAPEAPEX2-generated biotin-phenoxyl radicals cannot escape. For mapping of open cellular regions, we instead use a 'ratiometric' three-state SILACLACLAC protocol for high spatial specificity. Isotopic labeling of proteins takes 5-7 cell doublings. Generation of the biotinylated proteomic sample takes 1 d, acquiring the mass spectrometric data takes 2-5 d and analysis of the data to obtain the final proteomic list takes 1 week.
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