期刊
BIOACTIVE MATERIALS
卷 26, 期 -, 页码 452-464出版社
KEAI PUBLISHING LTD
DOI: 10.1016/j.bioactmat.2023.03.012
关键词
Cholangiocyte; Decellularized scaffold; Ductal organoid; Extracellular matrix
Developed functional ductal organoids (FDOs) with biliary tree networks in rat decellularized liver scaffolds (DLSs) using primary cholangiocytes from mouse bile ducts. FDOs showed morphological and functional characteristics of bile secretion and transportation, as well as the expression of specific biomarkers and the key molecule serotonin throughout the culture period. These FDOs may serve as valuable tools for disease modeling and drug screening in the field of liver regenerative medicine.
Developing functional ductal organoids (FDOs) is essential for liver regenerative medicine. We aimed to construct FDOs with biliary tree networks in rat decellularized liver scaffolds (DLSs) with primary cholangiocytes isolated from mouse bile ducts. The developed FDOs were dynamically characterized by functional assays and metabolomics for bioprocess clarification. FDOs were reconstructed in DLSs retaining native structure and bioactive factors with mouse primary cholangiocytes expressing enriched biomarkers. Morphological assessment showed that biliary tree-like structures gradually formed from day 3 to day 14. The cholangiocytes in FDOs maintained high viability and expressed 11 specific biomarkers. Basal-apical polarity was observed at day 14 with immunostaining for E-cadherin and acetylated alpha-tubulin. The rhodamine 123 transport assay and active collection of cholyl-lysyl-fluorescein exhibited the specific functions of bile secretion and transportation at day 14 compared to those in monolayer and hydrogel culture systems. The metabolomics analysis with 1075 peak pairs showed that serotonin, as a key molecule of the tryptophan metabolism pathway linked to biliary tree reconstruction, was specifically expressed in FDOs during the whole period of culture. Such FDOs with biliary tree networks and serotonin expression may be applied for disease modeling and drug screening, which paves the way for future clinical therapeutic applications.
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