期刊
BIOACTIVE MATERIALS
卷 26, 期 -, 页码 249-263出版社
KEAI PUBLISHING LTD
DOI: 10.1016/j.bioactmat.2023.03.002
关键词
peripheral nerves; chitosan oligosaccharides; fibroblast exosomes; TFAP2C; axon regeneration
In this study, the protein expression profiles in injured sciatic nerves were analyzed using proteomics. It was found that chitosan oligosaccharides (COS) up-regulate a group of proteins related to exosome packaging and transport, indicating the involvement of exosomes in COS-induced peripheral nerve regeneration. Exosomes derived from COS-treated fibroblasts promote axon extension and regeneration, and the key factor in this process is TFAP2C.
Chitosan and its degradation product, oligosaccharides, have been shown to facilitate peripheral nerve regeneration. However, the underlying mechanisms are not well understood. In this study, we analyzed the protein expression profiles in sciatic nerves after injury using proteomics. A group of proteins related to exosome packaging and transport is up-regulated by chitosan oligosaccharides (COS), implying that exosomes are involved in COS-induced peripheral nerve regeneration. In fact, exosomes derived from fibroblasts (f-EXOs) treated with COS significantly promoted axon extension and regeneration. Exosomal protein identification and functional studies, revealed that TFAP2C is a key factor in neurite outgrowth induced by COS-f-EXOs. Furthermore, we showed that TFAP2C targets the pri-miRNA-132 gene and represses miR-132-5p expression in dorsal root ganglion neurons. Camkk1 is a downstream substrate of miR-132-5p that positively affects axon extension. In rats, miR-132-5p antagomir stimulates CAMKK1 expression and improves axon regeneration and functional recovery in sciatic nerves after injury. Our data reveal the mechanism for COS in axon regeneration, that is COS induce fibroblasts to produce TFAP2C-enriched EXOs, which are then transferred into axons to promote axon regeneration via miR-132-5p/CAMKK1. Moreover, these results show a new facet of fibroblasts in axon regeneration in peripheral nerves.
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