期刊
BIOACTIVE MATERIALS
卷 28, 期 -, 页码 227-242出版社
KEAI PUBLISHING LTD
DOI: 10.1016/j.bioactmat.2023.05.013
关键词
Peripheral nerve regeneration; Bioceramics; Immuno-modulation microenvironment; Macrophage; Nerve guidance conduit
Biomaterials can enhance peripheral nerve regeneration by modulating the immune microenvironment and promoting SCs remyelination. In this study, inorganic Li-Mg-Si bioceramics were used to fabricate scaffolds that showed no cytotoxicity to SCs but promoted their migration and differentiation towards a remyelination state. The LMS-containing scaffolds also promoted macrophage polarization towards pro-regenerative M2-like cells, which facilitated SCs migration and differentiation. Implantation with LMS-containing nerve guidance conduits increased M2-like macrophage infiltration and enhanced nerve regeneration and motor functional recovery in a rat model of sciatic nerve injury.
Biomaterials can modulate the local immune and repair-supportive microenvironments to promote peripheral nerve regeneration. Inorganic bioceramics have been widely used for regulating tissue regeneration and local immune response. However, little is known on whether inorganic bioceramics can have potential for enhancing peripheral nerve regeneration and what are the mechanisms underlying their actions. Here, the inorganic lithium-magnesium-silicon (Li-Mg-Si, LMS) bioceramics containing scaffolds are fabricated and characterized. The LMS-containing scaffolds had no cytotoxicity against rat Schwann cells (SCs), but promoted their migration and differentiation towards a remyelination state by up-regulating the expression of neurotrophic factors in a & beta;-catenin-dependent manner. Furthermore, using single cell-sequencing, we showed that LMS-containing scaf-folds promoted macrophage polarization towards the pro-regenerative M2-like cells, which subsequently facil-itated the migration and differentiation of SCs. Moreover, implantation with the LMS-containing nerve guidance conduits (NGCs) increased the frequency of M2-like macrophage infiltration and enhanced nerve regeneration and motor functional recovery in a rat model of sciatic nerve injury. Collectively, these findings indicated that the inorganic LMS bioceramics offered a potential strategy for enhancing peripheral nerve regeneration by modulating the immune microenvironment and promoting SCs remyelination.
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