期刊
NATURE NANOTECHNOLOGY
卷 11, 期 9, 页码 798-807出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NNANO.2016.95
关键词
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资金
- National Institutes of Health (NIH) Director's New Innovator Award [1DP2OD007292]
- NIH Transformaive Research Award [1R01EB018659]
- Office of Naval Research (ONR) Young Investigator Program [N000141110914]
- ONR grants [N000141010827, N000141310593]
- National Science Foundation (NSF) Faculty Early Career Development Award [CCF1054898]
- NSF grant [CCF1162459]
- Wyss Institute for Biologically Engineering Faculty Startup Fund
- HHMI International Predoctoral Fellowship
- Alexander von Humboldt-Foundation through a Feodor-Lynen Fellowship
- NIH grant [5R21HD072481]
- Direct For Computer & Info Scie & Enginr
- Division of Computing and Communication Foundations [1317694] Funding Source: National Science Foundation
- Division of Computing and Communication Foundations
- Direct For Computer & Info Scie & Enginr [1054898] Funding Source: National Science Foundation
Recent advances in fluorescence super-resolution microscopy have allowed subcellular features and synthetic nanostructures down to 10-20 nm in size to be imaged. However, the direct optical observation of individual molecular targets (similar to 5 nm) in a densely packed biomolecular cluster remains a challenge. Here, we show that such discrete molecular imaging is possible using DNA-PAINT (points accumulation for imaging in nanoscale topography) a super-resolution fluorescence microscopy technique that exploits programmable transient oligonucleotide hybridization on synthetic DNA nanostructures. We examined the effects of a high photon count, high blinking statistics and an appropriate blinking duty cycle on imaging quality, and developed a software-based drift correction method that achieves <1 nm residual drift (root mean squared) over hours. This allowed us to image a densely packed triangular lattice pattern with similar to 5 nm point-to-point distance and to analyse the DNA origami structural offset with angstrom-level precision (2 A) from single-molecule studies. By combining the approach with multiplexed exchange-PAINT imaging, we further demonstrated an optical nanodisplay with 5 x 5 nm pixel size and three distinct colours with <1 nm cross-channel registration accuracy.
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