期刊
NATURE METHODS
卷 13, 期 4, 页码 325-328出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.3770
关键词
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资金
- US National Science Foundation
- US Department of Defense (National Defense Science and Engineering graduate fellowship)
- US National Institute of Mental Health (National Research Service Award postdoctoral fellowship) [1F32MH105053-01]
- German Academic Exchange Service (DAAD)
- Fidelity Foundation
- Japan Agency for Medical Research and Development
- Japan Society for the Promotion of Science (KAKENHI) [15K18372, 26115507, 15H02358]
- US National Institute on Drug Abuse
- US Army Research Laboratory and Defense Advanced Research Projects Agency [W911NF-14-2-0013]
- Grants-in-Aid for Scientific Research [15K18372, 15H02358, 26115507, 16H06276] Funding Source: KAKEN
Real-time activity measurements from multiple specific cell populations and projections are likely to be important for understanding the brain as a dynamical system. Here we developed frame-projected independent-fiber photometry (FIP), which we used to record fluorescence activity signals from many brain regions simultaneously in freely behaving mice. We explored the versatility of the FIP microscope by quantifying real-time activity relationships among many brain regions during social behavior, simultaneously recording activity along multiple axonal pathways during sensory experience, performing simultaneous two-color activity recording, and applying optical perturbation tuned to elicit dynamics that match naturally occurring patterns observed during behavior.
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