期刊
NATURE METHODS
卷 13, 期 1, 页码 24-27出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nmeth.3694
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资金
- US National Institute of General Medical Sciences
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [P01GM051487, R01GM063072] Funding Source: NIH RePORTER
Single-particle cryo-electron microscopy (cryo-EM) has emerged over the last two decades as a technique capable of studying challenging systems that otherwise defy structural characterization. Recent technical advances have resulted in a 'quantum leap' in applicability, throughput and achievable resolution that has gained this technique worldwide attention. Here I discuss some of the major historical landmarks in the development of the cryo-EM field, ultimately leading to its present success.
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