4.8 Review

Creating and evaluating accurate CRISPR-Cas9 scalpels for genomic surgery

相关参考文献

注意:仅列出部分参考文献,下载原文获取全部文献信息。
Article Biochemistry & Molecular Biology

Efficient Intracellular Delivery of Native Proteins

Diego S. D'Astolfo et al.

Article Biochemistry & Molecular Biology

Genome-wide CRISPR Screen in a Mouse Model of Tumor Growth and Metastasis

Sidi Chen et al.

Article Biochemistry & Molecular Biology

A Genome-wide CRISPR Screen in Primary Immune Cells to Dissect Regulatory Networks

Oren Parnas et al.

Article Biochemistry & Molecular Biology

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

Bernd Zetsche et al.

Article Cell Biology

A CRISPR/Cas9 Vector System for Tissue-Specific Gene Disruption in Zebrafish

Julien Ablain et al.

DEVELOPMENTAL CELL (2015)

Article Biochemistry & Molecular Biology

High-throughput gene targeting and phenotyping in zebrafish using CRISPR/Cas9

Gaurav K. Varshney et al.

GENOME RESEARCH (2015)

Article Biochemistry & Molecular Biology

Sequence determinants of improved CRISPR sgRNA design

Han Xu et al.

GENOME RESEARCH (2015)

Review Biotechnology & Applied Microbiology

Delivery and Specificity of CRISPR/Cas9 Genome Editing Technologies for Human Gene Therapy

Jennifer L. Gori et al.

HUMAN GENE THERAPY (2015)

Review Biotechnology & Applied Microbiology

The Bacterial Origins of the CRISPR Genome-Editing Revolution

Erik J. Sontheimer et al.

HUMAN GENE THERAPY (2015)

Article Biotechnology & Applied Microbiology

Dimeric CRISPR RNA-Guided FokI-dCas9 Nucleases Directed by Truncated gRNAs for Highly Specific Genome Editing

Nicolas Wyvekens et al.

HUMAN GENE THERAPY (2015)

Article Biochemistry & Molecular Biology

Convergence of Developmental and Oncogenic Signaling Pathways at Transcriptional Super-Enhancers

Denes Hnisz et al.

MOLECULAR CELL (2015)

News Item Multidisciplinary Sciences

CRISPR, THE DISRUPTOR

Heidi Ledford

NATURE (2015)

Article Multidisciplinary Sciences

Engineered CRISPR-Cas9 nucleases with altered PAM specificities

Benjamin P. Kleinstiver et al.

NATURE (2015)

Article Multidisciplinary Sciences

In vivo genome editing using Staphylococcus aureus Cas9

F. Ann Ran et al.

NATURE (2015)

Article Biotechnology & Applied Microbiology

Increasing the efficiency of precise genome editing with CRISPR-Cas9 by inhibition of nonhomologous end joining

Takeshi Maruyama et al.

NATURE BIOTECHNOLOGY (2015)

Article Biotechnology & Applied Microbiology

Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells

Ayal Hendel et al.

NATURE BIOTECHNOLOGY (2015)

Article Biotechnology & Applied Microbiology

Unbiased detection of off-target cleavage by CRISPR-Cas9 and TALENs using integrase-defective lentiviral vectors

Xiaoling Wang et al.

NATURE BIOTECHNOLOGY (2015)

Article Biotechnology & Applied Microbiology

Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells

Van Trung Chu et al.

NATURE BIOTECHNOLOGY (2015)

Letter Biotechnology & Applied Microbiology

A split-Cas9 architecture for inducible genome editing and transcription modulation

Bernd Zetsche et al.

NATURE BIOTECHNOLOGY (2015)

Article Biotechnology & Applied Microbiology

Discovery of cancer drug targets by CRISPR-Cas9 screening of protein domains

Junwei Shi et al.

NATURE BIOTECHNOLOGY (2015)

Article Biotechnology & Applied Microbiology

Photoactivatable CRISPR-Cas9 for optogenetic genome editing

Yuta Nihongaki et al.

NATURE BIOTECHNOLOGY (2015)

Article Biochemistry & Molecular Biology

Small molecule-triggered Cas9 protein with improved genome-editing specificity

Kevin M. Davis et al.

NATURE CHEMICAL BIOLOGY (2015)

Review Biochemistry & Molecular Biology

Therapeutic genome editing: prospects and challenges

David Benjamin Turitz Cox et al.

NATURE MEDICINE (2015)

Letter Biochemical Research Methods

Off-target mutations are rare in Cas9-modified mice

Vivek Iyer et al.

NATURE METHODS (2015)

Article Biochemical Research Methods

CRISPRscan: designing highly efficient sgRNAs for CRISPR-Cas9 targeting in vivo

Miguel A. Moreno-Mateos et al.

NATURE METHODS (2015)

Article Biochemical Research Methods

Unraveling CRISPR-Cas9 genome engineering parameters via a library-on-library approach

Raj Chari et al.

NATURE METHODS (2015)

Article Biochemical Research Methods

Digenome-seq: genome-wide profiling of CRISPR-Cas9 off-target effects in human cells

Daesik Kim et al.

NATURE METHODS (2015)

Review Microbiology

An updated evolutionary classification of CRISPR-Cas systems

Kira S. Makarova et al.

NATURE REVIEWS MICROBIOLOGY (2015)

Article Biochemistry & Molecular Biology

Structure and specificity of the RNA-guided endonuclease Cas9 during DNA interrogation, target binding and cleavage

Eric A. Josephs et al.

NUCLEIC ACIDS RESEARCH (2015)

Article Biochemistry & Molecular Biology

Efficient CRISPR-rAAV engineering of endogenous genes to study protein function by allele-specific RNAi

Manuel Kaulich et al.

NUCLEIC ACIDS RESEARCH (2015)

Article Biochemistry & Molecular Biology

Development of an intein-mediated split-Cas9 system for gene therapy

Dong-Jiunn Jeffery Truong et al.

NUCLEIC ACIDS RESEARCH (2015)

Article Biochemistry & Molecular Biology

Cas9-chromatin binding information enables more accurate CRISPR off-target prediction

Ritambhara Singh et al.

NUCLEIC ACIDS RESEARCH (2015)

Article Multidisciplinary Sciences

Generation of knock-in primary human T cells using Cas9 ribonucleoproteins

Kathrin Schumann et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2015)

Article Multidisciplinary Sciences

Rational design of a split-Cas9 enzyme complex

Addison V. Wright et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2015)

Article Multidisciplinary Sciences

A Cas9-guide RNA complex preorganized for target DNA recognition

Fuguo Jiang et al.

SCIENCE (2015)

Review Biotechnology & Applied Microbiology

Quantifying on- and off-target genome editing

Ayal Hendel et al.

TRENDS IN BIOTECHNOLOGY (2015)

Article Multidisciplinary Sciences

Somatic CRISPR/Cas9-mediated tumour suppressor disruption enables versatile brain tumour modelling

Marc Zuckermann et al.

NATURE COMMUNICATIONS (2015)

Article Biotechnology & Applied Microbiology

In vivo interrogation of gene function in the mammalian brain using CRISPR-Cas9

Lukasz Swiech et al.

NATURE BIOTECHNOLOGY (2015)

Article Biotechnology & Applied Microbiology

Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro and in vivo

John A. Zuris et al.

NATURE BIOTECHNOLOGY (2015)

Article Biotechnology & Applied Microbiology

GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases

Shengdar Q. Tsai et al.

NATURE BIOTECHNOLOGY (2015)

Article Biotechnology & Applied Microbiology

Genome-wide detection of DNA double-stranded breaks induced by engineered nucleases

Richard L. Frock et al.

NATURE BIOTECHNOLOGY (2015)

Article Biochemistry & Molecular Biology

Multi-kilobase homozygous targeted gene replacement in human induced pluripotent stem cells

Susan M. Byrne et al.

NUCLEIC ACIDS RESEARCH (2015)

Article Oncology

Applications of the CRISPR-Cas9 system in cancer biology

Francisco J. Sanchez-Rivera et al.

NATURE REVIEWS CANCER (2015)

Article Biochemical Research Methods

CasOT: a genome-wide Cas9/gRNA off-target searching tool

An Xiao et al.

BIOINFORMATICS (2014)

Article Biochemistry & Molecular Biology

CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling

Randall J. Platt et al.

Article Biochemistry & Molecular Biology

Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA

Hiroshi Nishimasu et al.

Article Cell & Tissue Engineering

Efficient Ablation of Genes in Human Hematopoietic Stem and Effector Cells using CRISPR/Cas9

Pankaj K. Mandal et al.

CELL STEM CELL (2014)

Editorial Material Cell & Tissue Engineering

What's Changed with Genome Editing?

Shengdar Q. Tsai et al.

CELL STEM CELL (2014)

Article Cell Biology

Global microRNA depletion suppresses tumor angiogenesis

Sidi Chen et al.

GENES & DEVELOPMENT (2014)

Article Biochemistry & Molecular Biology

Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases

Seung Woo Cho et al.

GENOME RESEARCH (2014)

Article Biochemistry & Molecular Biology

Gene disruption by cell-penetrating peptide-mediated delivery of Cas9 protein and guide RNA

Suresh Ramakrishna et al.

GENOME RESEARCH (2014)

Article Biochemistry & Molecular Biology

Chromosomal Translocations in Human Cells Are Generated by Canonical Nonhomologous End-Joining

Hind Ghezraoui et al.

MOLECULAR CELL (2014)

Article Multidisciplinary Sciences

Structural basis of PAM-dependent target DNA recognition by the Cas9 endonuclease

Carolin Anders et al.

NATURE (2014)

Article Multidisciplinary Sciences

Rapid modelling of cooperating genetic events in cancer through somatic genome editing

Francisco J. Sanchez-Rivera et al.

NATURE (2014)

Article Multidisciplinary Sciences

In vivo engineering of oncogenic chromosomal rearrangements with the CRISPR/Cas9 system

Danilo Maddalo et al.

NATURE (2014)

Article Multidisciplinary Sciences

CRISPR-mediated direct mutation of cancer genes in the mouse liver

Wen Xue et al.

NATURE (2014)

Article Multidisciplinary Sciences

DNA interrogation by the CRISPR RNA-guided endonuclease Cas9

Samuel H. Sternberg et al.

NATURE (2014)

Article Biotechnology & Applied Microbiology

Genome-wide recessive genetic screening in mammalian cells with a lentiviral CRISPR-guide RNA library

Hiroko Koike-Yusa et al.

NATURE BIOTECHNOLOGY (2014)

Article Biotechnology & Applied Microbiology

Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells

Xuebing Wu et al.

NATURE BIOTECHNOLOGY (2014)

Article Biotechnology & Applied Microbiology

Improving CRISPR-Cas nuclease specificity using truncated guide RNAs

Yanfang Fu et al.

NATURE BIOTECHNOLOGY (2014)

Article Biotechnology & Applied Microbiology

Dimeric CRISPR RNA-guided Fokl nucleases for highly specific genome editing

Shengdar Q. Tsai et al.

NATURE BIOTECHNOLOGY (2014)

Article Biotechnology & Applied Microbiology

Genome-wide analysis reveals characteristics of off-target sites bound by the Cas9 endonuclease

Cem Kuscu et al.

NATURE BIOTECHNOLOGY (2014)

Article Biotechnology & Applied Microbiology

Rational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation

John G. Doench et al.

NATURE BIOTECHNOLOGY (2014)

Article Biotechnology & Applied Microbiology

Fusion of catalytically inactive Cas9 to Fokl nuclease improves the specificity of genome modification

John P. Guilinger et al.

NATURE BIOTECHNOLOGY (2014)

Article Biotechnology & Applied Microbiology

Genome editing with Cas9 in adult mice corrects a disease mutation and phenotype

Hao Yin et al.

NATURE BIOTECHNOLOGY (2014)

Article Biochemical Research Methods

Adenoviral vector DNA for accurate genome editing with engineered nucleases

Maarten Holkers et al.

NATURE METHODS (2014)

Article Biochemical Research Methods

Efficient genome modification by CRISPR-Cas9 nickase with minimal off-target effects

Bin Shen et al.

NATURE METHODS (2014)

Letter Biochemical Research Methods

E-CRISP: fast CRISPR target site identification

Florian Heigwer et al.

NATURE METHODS (2014)

Article Biochemistry & Molecular Biology

Multiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector

Ami M. Kabadi et al.

NUCLEIC ACIDS RESEARCH (2014)

Article Biochemistry & Molecular Biology

CHOPCHOP: a CRISPR/Cas9 and TALEN web tool for genome editing

Tessa G. Montague et al.

NUCLEIC ACIDS RESEARCH (2014)

Article Biochemistry & Molecular Biology

Classification and evolution of type II CRISPR-Cas systems

Krzysztof Chylinski et al.

NUCLEIC ACIDS RESEARCH (2014)

Article Multidisciplinary Sciences

Direct observation of R-loop formation by single RNA-guided Cas9 and Cascade effector complexes

Mark D. Szczelkun et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2014)

Article Biochemistry & Molecular Biology

Efficient in vivo deletion of a large imprinted lncRNA by CRISPR/Cas9

Jinxiong Han et al.

RNA BIOLOGY (2014)

Article Multidisciplinary Sciences

Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells

Ophir Shalem et al.

SCIENCE (2014)

Article Multidisciplinary Sciences

Genetic Screens in Human Cells Using the CRISPR-Cas9 System

Tim Wang et al.

SCIENCE (2014)

Review Multidisciplinary Sciences

The new frontier of genome engineering with CRISPR-Cas9

Jennifer A. Doudna et al.

SCIENCE (2014)

Article Multidisciplinary Sciences

Structures of Cas9 Endonucleases Reveal RNA-Mediated Conformational Activation

Martin Jinek et al.

SCIENCE (2014)

Article Multidisciplinary Sciences

Engineering human tumour-associated chromosomal translocations with the RNA-guided CRISPR-Cas9 system

R. Torres et al.

NATURE COMMUNICATIONS (2014)

Article Multidisciplinary Sciences

Comparison of non-canonical PAMs for CRISPR/Cas9-mediated DNA cleavage in human cells

Yilan Zhang et al.

SCIENTIFIC REPORTS (2014)

Article Medicine, Research & Experimental

COSMID: A Web-based Tool for Identifying and Validating CRISPR/Cas Off-target Sites

Thomas J. Cradick et al.

MOLECULAR THERAPY-NUCLEIC ACIDS (2014)

Article Biochemistry & Molecular Biology

Dynamic Imaging of Genomic Loci in Living Human Cells by an Optimized CRISPR/Cas System

Baohui Chen et al.

Article Biochemistry & Molecular Biology

Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity

F. Ann Ran et al.

Article Biochemistry & Molecular Biology

Processing-Independent CRISPR RNAs Limit Natural Transformation in Neisseria meningitidis

Yan Zhang et al.

MOLECULAR CELL (2013)

Article Biotechnology & Applied Microbiology

CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering

Prashant Mali et al.

NATURE BIOTECHNOLOGY (2013)

Article Biotechnology & Applied Microbiology

DNA targeting specificity of RNA-guided Cas9 nucleases

Patrick D. Hsu et al.

NATURE BIOTECHNOLOGY (2013)

Article Biotechnology & Applied Microbiology

Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease

Seung Woo Cho et al.

NATURE BIOTECHNOLOGY (2013)

Article Biotechnology & Applied Microbiology

Efficient genome editing in zebrafish using a CRISPR-Cas system

Woong Y. Hwang et al.

NATURE BIOTECHNOLOGY (2013)

Article Biotechnology & Applied Microbiology

High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity

Vikram Pattanayak et al.

NATURE BIOTECHNOLOGY (2013)

Article Biotechnology & Applied Microbiology

High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells

Yanfang Fu et al.

NATURE BIOTECHNOLOGY (2013)

Article Biochemical Research Methods

Nucleotide-resolution DNA double-strand break mapping by next-generation sequencing

Nicola Crosetto et al.

NATURE METHODS (2013)

Article Biochemical Research Methods

Orthogonal Cas9 proteins for RNA-guided gene regulation and editing

Kevin M. Esvelt et al.

NATURE METHODS (2013)

Review Cell Biology

INNOVATION TALENs: a widely applicable technology for targeted genome editing

J. Keith Joung et al.

NATURE REVIEWS MOLECULAR CELL BIOLOGY (2013)

Article Biochemistry & Molecular Biology

Chromosomal deletions and inversions mediated by TALENs and CRISPR/Cas in zebrafish

An Xiao et al.

NUCLEIC ACIDS RESEARCH (2013)

Article Biochemistry & Molecular Biology

Optimization of scarless human stem cell genome editing

Luhan Yang et al.

NUCLEIC ACIDS RESEARCH (2013)

Article Multidisciplinary Sciences

Heritable and Precise Zebrafish Genome Editing Using a CRISPR-Cas System

Woong Y. Hwang et al.

PLOS ONE (2013)

Article Multidisciplinary Sciences

Efficient multiplex biallelic zebrafish genome editing using a CRISPR nuclease system

Li-En Jao et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2013)

Article Multidisciplinary Sciences

Efficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis

Zhonggang Hou et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2013)

Article Multidisciplinary Sciences

RNA-Guided Human Genome Engineering via Cas9

Prashant Mali et al.

SCIENCE (2013)

Article Multidisciplinary Sciences

Multiplex Genome Engineering Using CRISPR/Cas Systems

Le Cong et al.

SCIENCE (2013)

Article Biology

RNA-programmed genome editing in human cells

Martin Jinek et al.

Article Multidisciplinary Sciences

In vivo genome editing using a high-efficiency TALEN system

Victoria M. Bedell et al.

NATURE (2012)

Article Multidisciplinary Sciences

Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria

Giedrius Gasiunas et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2012)

Article Multidisciplinary Sciences

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

Martin Jinek et al.

SCIENCE (2012)

Article Biotechnology & Applied Microbiology

An unbiased genome-wide analysis of zinc-finger nuclease specificity

Richard Gabriel et al.

NATURE BIOTECHNOLOGY (2011)

Article Biotechnology & Applied Microbiology

Effect of Genome Size on AAV Vector Packaging

Zhijian Wu et al.

MOLECULAR THERAPY (2010)

Review Genetics & Heredity

Genome editing with engineered zinc finger nucleases

Fyodor D. Urnov et al.

NATURE REVIEWS GENETICS (2010)

Article Biochemistry & Molecular Biology

ZiFiT (Zinc Finger Targeter): an updated zinc finger engineering tool

Jeffry D. Sander et al.

NUCLEIC ACIDS RESEARCH (2010)

Article Medicine, Research & Experimental

Insertional oncogenesis in 4 patients after retrovirus-mediated gene therapy of SCID-X1

Salima Hacein-Bey-Abina et al.

JOURNAL OF CLINICAL INVESTIGATION (2008)