期刊
NATURE METHODS
卷 13, 期 1, 页码 41-50出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.3684
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资金
- US National Institutes of Health [R01AI117839, R01HL093766]
- NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [R01HL093766] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [R01AI117839] Funding Source: NIH RePORTER
The simplicity of site-specific genome targeting by type II clustered, regularly interspaced, short palindromic repeat (CRISPR)-Cas9 nucleases, along with their robust activity profile, has changed the landscape of genome editing. These favorable properties have made the CRISPR-Cas9 system the technology of choice for sequence-specific modifications in vertebrate systems. For many applications, whether the focus is on basic science investigations or therapeutic efficacy, activity and precision are important considerations when one is choosing a nuclease platform, target site and delivery method. Here we review recent methods for increasing the activity and accuracy of Cas9 and assessing the extent of off-target cleavage events.
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