期刊
NATURE METHODS
卷 13, 期 11, 页码 928-+出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nmeth.4029
关键词
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资金
- Michigan State University under the Training Program in Plant Biotechnology for Health and Sustainability [T32-GM110523]
- Howard Hughes Medical Institute Gilliam fellowship
- US Department of Agriculture National Institute of Food and Agriculture award [2016-67011-24701]
- US National Science Foundation Career Award CBET [1254238]
- Directorate For Engineering
- Div Of Chem, Bioeng, Env, & Transp Sys [1254238] Funding Source: National Science Foundation
- NIFA [2016-67011-24701, 810668] Funding Source: Federal RePORTER
Deep mutational scanning is a foundational tool for addressing the functional consequences of large numbers of mutants, but a more efficient and accessible method for construction of user-defined mutagenesis libraries is needed. Here we present nicking mutagenesis, a robust, single-day, one-pot saturation mutagenesis method performed on routinely prepped plasmid dsDNA. The method can be used to produce comprehensive or single-or multi-site saturation mutagenesis libraries.
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