Evaluation of the Effects of e-Cigarette Aerosol Extracts and Tobacco Cigarette Smoke Extracts on RAW264.7 Cells

With the advancement of society, electronic cigarettes(e-cigarettes)have gained popularity among a growing number of individuals. Whilenumerous toxicological studies have suggested that e-cigarettes area safer alternative to traditional cigarettes, there is also a bodyof literature presenting contrasting findings. This in vitro study aimed to compare the effects of e-cigarettes and tobacco cigarettes(t-cigarettes) on RAW264.7 cells by using four e-cigarette aerosolextracts (ECA) and cigarette smoking extracts (CS) containing differentnicotine concentrations. The results revealed that low concentrationof nicotine in CS as well as in ECA with grape, watermelon, and colaflavors could promote cell viability. Conversely, high nicotine concentrationin CS and ECA with four flavors decreased cell viability. Furthermore,our study demonstrated that CS significantly reduced the phagocyticcapability of RAW264.7 cells and increased the levels of inflammatorycytokines (IL-6, TNF-& alpha;, and IL-1 & beta;) and reactive oxygenspecies (ROS) compared to ECA. Overall, our findings indicate allfour e-cigarettes induced less cytotoxicity to RAW264.7 cells andmight be safer than t-cigarettes.

Automated summary

With the progression of society, e-cigarettes have become popular among many people. While some studies suggest that e-cigarettes are a safer alternative to traditional cigarettes, there are conflicting findings. This in vitro study compared the effects of e-cigarettes and tobacco cigarettes on RAW264.7 cells. The results showed that low nicotine concentration and certain flavors in e-cigarettes promoted cell viability, while high nicotine concentration and certain flavors decreased cell viability. Additionally, tobacco cigarettes reduced the phagocytic capability of cells and increased inflammation and oxidative stress compared to e-cigarettes.