期刊
NATURE METHODS
卷 13, 期 2, 页码 127-137出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.3733
关键词
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资金
- US National Institutes of Health (NIH) [R01DA036865, U01HG007900, R21AR065956, P30AR066527]
- NIH Director's New Innovator Award [DP20D008586]
- US National Science Foundation (NSF) Faculty Early Career Development (CAREER) award [CBET-1151035]
- NSF Graduate Research Fellowship
- American Heart Association Mid-Atlantic Affiliate Predoctoral Fellowship
- NIH biotechnology training grant [T32GM008555]
- Directorate For Engineering
- Div Of Chem, Bioeng, Env, & Transp Sys [1151035] Funding Source: National Science Foundation
Gene regulation is a complex and tightly controlled process that defines cell identity, health and disease, and response to pharmacologic and environmental signals. Recently developed DNA-targeting platforms, including zinc finger proteins, transcription activator-like effectors (TALEs) and the clustered, regularly interspaced, short palindromic repeats (CRISPR)-Cas9 system, have enabled the recruitment of transcriptional modulators and epigenome-modifying factors to any genomic site, leading to new insights into the function of epigenetic marks in gene expression. Additionally, custom transcriptional and epigenetic regulation is facilitating refined control over cell function and decision making. The unique properties of the CRISPR-Cas9 system have created new opportunities for high-throughput genetic screens and multiplexing targets to manipulate complex gene expression patterns. This Review summarizes recent technological developments in this area and their application to biomedical challenges. We also discuss remaining limitations and necessary future directions for this field.
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