☆ 4.6 Article

Dissecting the Permeability of the Escherichia coli Cell Envelope to a Small Molecule Using Tailored Intensiometric Fluorescent Protein Sensors

ACS OMEGA (2023)

相关参考文献

注意：仅列出部分参考文献，下载原文获取全部文献信息。

Review Chemistry, Multidisciplinary

FhuA: From Iron-Transporting Transmembrane Protein to Versatile Scaffolds through Protein Engineering

Daniel F. Sauer et al.

Summary: Protein engineering is a powerful method for tailoring protein properties, enabling the design of biohybrid catalysts and materials. The ferric hydroxamate uptake protein FhuA has been used as a versatile scaffold with a large cavity and stability towards temperature and organic solvents. Applications of FhuA have been explored in biocatalysis, materials science, and the construction of artificial metalloenzymes, showcasing its potential for creating hybrid catalysts and materials.

ACCOUNTS OF CHEMICAL RESEARCH (2023)

添加到收藏夹

Article Biochemistry & Molecular Biology

Ratiometric Bioluminescent Zinc Sensor Proteins to Quantify Serum and Intracellular Free Zn2+

Claire M. S. Michielsen et al.

Summary: This study expanded the toolbox of bioluminescent Zn2+ sensors by developing two new sensor families that show a large change in the emission ratio and cover a range of physiologically relevant Zn2+ affinities, providing an attractive alternative to fluorescence sensors for applications in complex media.

ACS CHEMICAL BIOLOGY (2022)

添加到收藏夹

Article Biochemical Research Methods

Functional Nanopore Screen: A Versatile High-Throughput Assay to Study and Engineer Protein Nanopores in Escherichia coli

Wadim Weber et al.

Summary: Nanopores are versatile membrane proteins that play key roles in physiology and have various biotechnological applications. However, the study and engineering of protein nanopores have been limited by a lack of suitable assays. The functional nanopore (FuN) screen provides a quantitative and dynamic readout of nanopore assembly and function using genetically encoded fluorescent protein sensors. This method has potential applications in fundamental studies and complex nanopore engineering in fields such as biomedicine, biotechnology, and synthetic biology.

ACS SYNTHETIC BIOLOGY (2022)

添加到收藏夹

Article Biochemical Research Methods

iFLinkC-X: A Scalable Framework to Assemble Bespoke Genetically Encoded Co-polymeric Linkers of Variable Lengths and Amino Acid Composition

Alexander Graewe et al.

Summary: Linker engineering is gaining importance in protein engineering and synthetic biology as scientists construct sophisticated protein assemblies for various applications. However, there is a lack of effective methods to code linkers and tailor them to the functional requirements of different fusion proteins. A scalable framework is proposed to compose customizable linkers based on a limited number of linker fragments stored in sequence-verified entry plasmids.

BIOCONJUGATE CHEMISTRY (2022)

添加到收藏夹

Article Biochemistry & Molecular Biology

The present and the future of protein biosensor engineering

Colin Jackson et al.

Summary: Protein biosensors are crucial in cell and neurobiology and have the potential to transform clinical and industrial analytics. The shift from multicomponent biosensors to single chain allosteric biosensors has brought them closer to commercial applications. Constructing artificial receptors can overcome the scarcity of natural receptors with sufficient conformational changes to control allosteric reporter domains.

CURRENT OPINION IN STRUCTURAL BIOLOGY (2022)

添加到收藏夹

Article Biochemistry & Molecular Biology

Structure- and mechanism-guided design of single fluorescent protein-based biosensors

Yusuke Nasu et al.

Summary: Genetically encoded biosensors based on fluorescence modulation of a single protein have been powerful imaging tools for monitoring neural activities. The GCaMP series of Ca2+ biosensors has been steadily improving for the past two decades and has become essential for neuroscience. Other biosensors have not reached the same performance levels or revolutionary impacts in specific disciplines as the Ca2+ biosensors. By understanding the structures and mechanisms of biosensors, guidelines have been provided to accelerate the development of a broader range of high-performance imaging tools.

NATURE CHEMICAL BIOLOGY (2021)

添加到收藏夹

Review Microbiology

Porins and small-molecule translocation across the outer membrane of Gram-negative bacteria

Julia Vergalli et al.

NATURE REVIEWS MICROBIOLOGY (2020)

添加到收藏夹

Article Biochemistry & Molecular Biology

iFLinkC: an iterative functional linker cloning strategy for the combinatorial assembly and recombination of linker peptides with functional domains

Alexander Graewe et al.

NUCLEIC ACIDS RESEARCH (2020)

添加到收藏夹

Review Biochemical Research Methods

Transporter engineering in microbial cell factories: the ins, the outs, and the in-betweens

Steven A. van der Hoek et al.

CURRENT OPINION IN BIOTECHNOLOGY (2020)

添加到收藏夹

Review Biotechnology & Applied Microbiology

Nanomedicine approaches for sirolimus delivery: a review of pharmaceutical properties and preclinical studies

Azadeh Haeri et al.

ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY (2018)

添加到收藏夹

Article Biochemistry & Molecular Biology

Evolution of a split RNA polymerase as a versatile biosensor platform

Jinyue Pu et al.

NATURE CHEMICAL BIOLOGY (2017)

添加到收藏夹

Review Biophysics

Structural Biology of the Major Facilitator Superfamily Transporters

Nieng Yan

ANNUAL REVIEW OF BIOPHYSICS, VOL 44 (2015)

添加到收藏夹

Review Cell Biology

Rapamycin: One Drug, Many Effects

Jing Li et al.

CELL METABOLISM (2014)

添加到收藏夹

Article Multidisciplinary Sciences

Protease-based synthetic sensing and signal amplification

Viktor Stein et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2014)

添加到收藏夹

Article Microbiology

Bioluminescence Resonance Energy Transfer System for Measuring Dynamic Protein-Protein Interactions in Bacteria

Boyu Cui et al.

MBIO (2014)

添加到收藏夹

Article Biochemistry & Molecular Biology

Engineered Luciferase Reporter from a Deep Sea Shrimp Utilizing a Novel Imidazopyrazinone Substrate

Mary P. Hall et al.

ACS CHEMICAL BIOLOGY (2012)

添加到收藏夹

Review Biochemistry & Molecular Biology

Protein translocation as a tool: The current rapamycin story

Mateusz Putyrski et al.

FEBS LETTERS (2012)

添加到收藏夹

Article Biochemistry & Molecular Biology

Small-Molecule Control of Protein Degradation Using Split Adaptors

Joseph H. Davis et al.

ACS CHEMICAL BIOLOGY (2011)

添加到收藏夹

Article Microbiology

Using Superfolder Green Fluorescent Protein for Periplasmic Protein Localization Studies

Thuy Dinh et al.

JOURNAL OF BACTERIOLOGY (2011)

添加到收藏夹

Article Biochemistry & Molecular Biology

Redesign of a Plugged β-Barrel Membrane Protein

Mohammad M. Mohammad et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2011)

添加到收藏夹

Article Biotechnology & Applied Microbiology

A propionate-inducible expression system for enteric bacteria

SK Lee et al.

APPLIED AND ENVIRONMENTAL MICROBIOLOGY (2005)

添加到收藏夹

Article Chemistry, Multidisciplinary

Characterization of the FKBP•Rapamycin•FRB ternary complex

LA Banaszynski et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2005)

添加到收藏夹

© Peeref 2019-2024. All rights reserved.