期刊
PLANTS-BASEL
卷 12, 期 21, 页码 -出版社
MDPI
DOI: 10.3390/plants12213768
关键词
high-affinity K+ transporter (HKT); triangle of U; Brassica napus L.; abiotic stress; heavy-metal tolerance
This study identified and characterized HKT genes in rapeseed, revealing their roles in response to abiotic stresses. Based on phylogenetic relationships and functional domain composition, the HKT members were classified into four subgroups. The expression profiles of BnaHKT genes varied under potassium, phytohormone, and heavy-metal treatment, suggesting their involvement in heavy-metal tolerance.
Members of the high-affinity potassium transporter (HKT) protein family regulate the uptake and homeostasis of sodium and potassium ions, but little research describes their roles in response to abiotic stresses in rapeseed (Brassica napus L.). In this study, we identified and characterized a total of 36 HKT genes from the species comprising the triangle of U model (U-triangle species): B. rapa, B. nigra, B. oleracea, B. juncea, B. napus, and B. carinata. We analyzed the phylogenetic relationships, gene structures, motif compositions, and chromosomal distributions of the HKT family members of rapeseed. Based on their phylogenetic relationships and assemblage of functional domains, we classified the HKT members into four subgroups, HKT1;1 to HKT1;4. Analysis of the nonsynonymous substitutions (Ka), synonymous substitutions (Ks), and the Ka/Ks ratios of HKT gene pairs suggested that these genes have experienced strong purifying selective pressure after duplication, with their evolutionary relationships supporting the U-triangle theory. Furthermore, the expression profiles of BnaHKT genes varies among potassium, phytohormone and heavy-metal treatment. Their repression provides resistance to heavy-metal stress, possibly by limiting uptake. Our results systematically reveal the characteristics of HKT family proteins and their encoding genes in six Brassica species and lay a foundation for further exploration of the role of HKT family genes in heavy-metal tolerance.
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