Phenotypic Diversity in Leaf Cuticular Waxes in Brassica carinata Accessions

Brassica carinata has received considerable attention as a renewable biofuel crop for semi-arid zones due to its high oil content and polyunsaturated fatty acids contents. It is important to develop new drought-resistant cultivars of B. carinata production to expand its areas into more arid regions. The accumulation of leaf cuticular wax on plant surfaces is one mechanism that reduces non-stomatal water loss, thus increasing drought resistance in plants. To explore phenotypic variations in cuticular wax in B. carinata, leaf waxes were extracted and quantified from a diversity panel consisting of 315 accessions. The results indicate that the accessions have a wide range of total leaf wax content (289-1356 mu g dm(-2)), wax classes, and their components. The C-29 and C-31 homologues of alkanes, C-29 ketone homologue, C-29 secondary alcohol, and C-30 aldehyde were the most abundant leaf waxes extracted from B. carinata accessions. The high heritability values of these waxes point to the positive selection for high wax content during early generations of future B. carinata breeding programs. Positive correlation coefficients, combined with the effects of these waxes on leaf wax content accumulation, suggest that modifying specific wax content could increase the total wax content and enhance cuticle composition. The identified leaf wax content and compositions in B. carinata will lead to the future discovery of wax biosynthetic pathways, the dissection of its genetic regulatory networks, the identification of candidate genes controlling production of these waxes, and thus, develop and release new B. carinata drought-tolerant cultivars.

Automated summary

Brassica carinata is a renewable biofuel crop with high oil and polyunsaturated fatty acids contents. This study explores the variations in cuticular wax content and composition in B. carinata, revealing a wide range of wax content and various classes of wax. The study also suggests that modifying specific wax content can increase the overall wax content and enhance the composition of the cuticle. This research provides valuable insight into wax biosynthetic pathways and the development of drought-tolerant cultivars of B. carinata.