Exogenous Hydrogen Sulfide Supplementation Alleviates the Salinity-Stress-Mediated Growth Decline in Wheat (Triticum aestivum L.) by Modulating Tolerance Mechanisms

The impact of the exogenous supplementation of hydrogen sulfide (20 and 50 mu M HS) on growth, enzyme activity, chlorophyll pigments, and tolerance mechanisms was studied in salinity-stressed (100 mM NaCl) wheat. Salinity significantly reduced height, fresh and dry weight, chlorophyll, and carotenoids. However, the supplementation of HS (at both concentrations) increased these attributes and also mitigated the decline to a considerable extent. The exogenous supplementation of HS reduced the accumulation of hydrogen peroxide (H2O2) and methylglyoxal (MG), thereby reducing lipid peroxidation and increasing the membrane stability index (MSI). Salinity stress increased H2O2, MG, and lipid peroxidation while reducing the MSI. The activity of nitrate reductase was reduced due to NaCl. However, the supplementation of HS alleviated the decline with obvious effects being seen due to 50 mu M HS. The activity of antioxidant enzymes (superoxide dismutase, catalase, ascorbate peroxidase, and glutathione reductase) was assayed and the content of reduced glutathione (GSH) increased due to salt stress and the supplementation of HS further enhanced their activity. A decline in ascorbic acid due to salinity stress was alleviated due to HS treatment. HS treatment increased the endogenous concentration of HS and nitric oxide (NO) under normal conditions. However, under salinity stress, HS supplementation resulted in a reduction in HS and NO as compared to NaCl-treated plants. In addition, proline and glycine betaine increased due to HS supplementation. HS treatment reduced sodium levels, while the increase in potassium justified the beneficial role of applied HS in improving salt tolerance in wheat.

Automated summary

This study investigated the impact of exogenous supplementation of hydrogen sulfide (HS) on the growth, enzyme activity, chlorophyll pigments, and tolerance mechanisms in salinity-stressed wheat. The results showed that HS supplementation increased the growth attributes of wheat and mitigated the decline caused by salt stress. HS supplementation also enhanced antioxidant enzyme activity, reduced lipid peroxidation, and increased the content of reduced glutathione. Furthermore, HS supplementation increased the levels of proline and glycine betaine, reduced sodium levels, and increased potassium levels. However, under salt stress, HS supplementation led to a reduction in hydrogen sulfide and nitric oxide levels.