期刊
PLANTS-BASEL
卷 12, 期 21, 页码 -出版社
MDPI
DOI: 10.3390/plants12213678
关键词
taxonomic assignment; QIIME2; ASV-specific database; pre-trained classifiers; public reference databases
The taxonomic assignment of sequences obtained through high throughput amplicon sequencing is limited for various applications. To address this issue, a new tool called ASVmaker has been developed to create custom databases and improve the accuracy of specific identifications.
The taxonomic assignment of sequences obtained by high throughput amplicon sequencing poses a limitation for various applications in the biomedical, environmental, and agricultural fields. Identifications are constrained by the length of the obtained sequences and the computational processes employed to efficiently assign taxonomy. Arriving at a consensus is often preferable to uncertain identification for ecological purposes. To address this issue, a new tool called ASVmaker has been developed to facilitate the creation of custom databases, thereby enhancing the precision of specific identifications. ASVmaker is specifically designed to generate reference databases for allocating amplicon sequencing data. It uses publicly available reference data and generates specific sequences derived from the primers used to create amplicon sequencing libraries. This versatile tool can complete taxonomic assignments performed with pre-trained classifiers from the SILVA and UNITE databases. Moreover, it enables the generation of comprehensive reference databases for specific genes in cases where no directly applicable database exists for taxonomic classification tools.
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