期刊
NATURE METHODS
卷 13, 期 10, 页码 852-+出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nmeth.3972
关键词
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资金
- Marie-Curie IntraEuropean Fellowship
- MRC [MC_U105178790]
- Medical Research Council [MC_U105178790] Funding Source: researchfish
- MRC [MC_U105178790] Funding Source: UKRI
We present tRNA-based vectors for producing multiple clustered regularly interspaced short palindromic repeats (CRISPR) single guide RNAs (sgRNAs) from a single RNA polymerase II or III transcript in Drosophila. The system, which is based on liberation of sgRNAs by processing flanking tRNAs, permits highly efficient multiplexing of Cas9-based mutagenesis. We also demonstrate that the tRNA-sgRNA system markedly increases the efficacy of conditional gene disruption by Cas9 and can promote editing by the recently discovered RNA-guided endonuclease Cpf1.
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