Green synthesis, characterization of silver nanoparticles using aqueous leaf extracts of Solanum melongena and in vitro evaluation of antibacterial, pesticidal and anticancer activity in human MDA-MB-231 breast cancer cell lines

The present study validated the green synthesis and characterization of AgNPs using the leaf extracts of Solanum melongena (SM-AgNPs). The efficiency of the phytocompounds in the leaf extract in reducing Ag+ to Ag0 ions was studied. The synthesized SM-AgNPs were characterized by spectroscopic methods and standard methods were adopted for the antioxidant activity, HPTLC, cytotoxicity and antimicrobial assays and pesticidal activity as well. The synthesised nanoparticle was measured to be 20.54 nm which was confirmed by the characterization. The total phenolic content was 5.98 & PLUSMN; 0. GAE/g which indicated potent antioxidant activity. HPTLC profile showed thirteen peaks corresponding to the phenolic compounds with Rfvalues ranging from 0.05 to 0.95. The average particle size and zeta potential was 75.14 nm-19.8 mV respectively. Cytotoxicity assay of the synthesized AgNP's showed viability percentage of 50.23 at 100 mg/ mL. Treatment of MDA-MB-231 cell lines with the SM-AgNPs induced early apoptosis in a greater number of cells. The synthesizedAgNPs were effective against the bacterial isolates Escherichia coli, Pseudomonas aeruginosa, Shigella flexneri, Proteus vulgaris, Klebsiella pneumoniae and Staphylococcus aureus. High mortal-ity of Bemisiatabaci was observed in the100 lg/mL of the SM-AgNPs treated concentrations. The study demonstrated the efficiency of SM-AgNPs against cancer cell lines, microbial isolates and insect pests.& COPY; 2023 The Author(s). Published by Elsevier B.V. on behalf of King Saud University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

Automated summary

This study validated the green synthesis and characterization of SM-AgNPs using leaf extracts, and investigated its efficiency in reducing Ag+ to Ag0 ions. The synthesized SM-AgNPs were characterized and tested for their antioxidant activity, HPTLC, cytotoxicity, antimicrobial assays, and pesticidal activity. The study demonstrated the effectiveness of SM-AgNPs against cancer cells, microbial isolates, and insect pests.